Reversed-phase ion-pair partition chromatography of carboxylates and sulphonates

Journal of Chromatography
K G Wahlung

Abstract

A reversed-phase partition chromatographic system for separation of organic anions as ion pairs with quaternary ammonium ions has been developed. Commercial, hydrophobized silica supports are used with 1-pentanol as stationary phase and aqueous solutions of tetrabutylammonium (TBA) as mobile phase. The separation of the aromatic sulphonates and benzoic acid derivatives is demonstrated. The use of the TBA concentration of the mobile phase to regulate the capacity factor of the anions, as a means of improving separation by gradient elution and direct injection of large sample volumes, is demonstrated. The isolation of nicotinic acid from human serum samples is shown.

Citations

Apr 1, 1977·Antimicrobial Agents and Chemotherapy·J P Anhalt
Feb 10, 2004·Applied and Environmental Microbiology·Jürgen MaierGeorg M Gübitz
Jul 7, 1976·Journal of Chromatography·B A Persson, P O Lagerström

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