Sep 1, 1976

Reversed-phase liquid-chromatographic analysis of hemodialysate from uremic patients

Clinical Chemistry
F C SenftleberD A Dayton

Abstract

With reversed-phase high-performance liquid chromatography we effected rapid and efficient separation of metabolites present in hemodialysate fluid from uremic patients on the artificial kidney. With an aqueous sodium acetate/methanol gradient as mobile phase, more than 50 ultraviolet-absorbing constituents were resolved in a 70-min chromatogram of a 100-mul sample of dialysate. Many of the metabolites could be detected in concentrations below 0.1 mg/liter. From results of ultraviolet spectrophotometric analysis and the gas- and liquid-chromatographic properties of standards, half the observed chromatographic peaks have been characterized or identified. The method has been shown to be more than 10-fold faster than comparable separations by ion-exchange techniques.

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Mentioned in this Paper

Hemodialysis
Dialysis Solutions
Separation Anxiety Disorder of Childhood
Uremia
Spectrophotometry, Ultraviolet
Kidney Failure, Chronic
High Pressure Liquid Chromatography Procedure
Chromatography, Gas-Liquid
Hemolytic-Uremic Syndrome
Metabolite

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