PMID: 9434112Jan 20, 1998Paper

Reversible activation of a cryptic cleavage site within E. coli beta-galactosidase in beta-galactosidase fusion proteins

Biochimica Et Biophysica Acta
E ViaplanaA Villaverde

Abstract

The VP60 capsid protein of rabbit haemorrhagic disease virus (60 kDa) has been fused to the C-terminus of beta-galactosidase and produced in E. coli from two related expression vectors. One of these vectors, carries a 429 bp DNA segment encoding the N-terminus peptide of VP60, and directs the synthesis of a larger fusion that contains the entire viral protein. Both fusion proteins are efficiently cleaved at a presumed trypsin-like target site within the carboxy moiety of beta-galactosidase (Arg 611-Thr 612), which is activated by the presence of the viral partner. In the larger fusion, VP60 is released by a cleavage within the linker region that affects about 10% of the chimeric proteins. In this situation, the resulting beta-galactosidase-like fragment recovers its natural proteolytic stability. These results prove that cryptic cleavage sites in beta-galactosidase can be efficiently activated in a fusion protein and suggest that this activation is based on reversible steric constraints generated by the fusion partner.

References

Mar 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Ullmann
Sep 1, 1992·Trends in Biotechnology·S O Enfors
Dec 1, 1992·Scandinavian Journal of Primary Health Care·U WrejeH Aberg
Feb 15, 1992·Experientia·M R Maurizi
Sep 1, 1989·FEMS Microbiology Reviews·A M Lazdunski
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Nov 15, 1986·The Biochemical Journal·F A Marston
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
May 1, 1994·Protein Science : a Publication of the Protein Society·S J HubbardJ M Thornton
Jun 30, 1994·Nature·R H JacobsonB W Matthews
Jun 15, 1991·Physical Review. a·J B SwiftG Ahlers

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Citations

Jun 13, 2001·Biochemical and Biophysical Research Communications·R CubarsíA Villaverde
Oct 29, 2015·Molecular Microbiology·Samuel M D OliveiraAndre S Ribeiro
Apr 13, 2000·FEBS Letters·M M CarrióA Villaverde
Sep 17, 1998·FEBS Letters·J X Feliu, A Villaverde
Dec 16, 1998·FEMS Microbiology Letters·M M CarrióA Villaverde
Jul 23, 1999·Biotechnology and Bioengineering·J L Corchero, A Villaverde

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