PMID: 2106680Mar 1, 1990Paper

Reversible ADP-ribosylation is demonstrated to be a regulatory mechanism in prokaryotes by heterologous expression

Proceedings of the National Academy of Sciences of the United States of America
H FuG P Roberts

Abstract

The primary product of biological nitrogen fixation, ammonia, reversibly regulates nitrogenase activity in a variety of diazotrophs by a process called "NH4(+)-switch-off/on." Strong correlative evidence from work in Azospirillum lipoferum and Rhodospirillum rubrum indicates that this regulation involves both the inactivation of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase and the reactivation by dinitrogenase reductase activating glycohydrolase. The genes encoding these two enzymes, draT and draG, have been cloned from these two organisms, so that direct genetic evidence can be marshaled to test this model in vivo. The draT/G system has been transferred to and monitored in the enteric nitrogen-fixing bacterium Klebsiella pneumoniae, an organism normally devoid of such a regulatory mechanism. The expressed draT and draG genes allowed K. pneumoniae to respond to NH4Cl with a reversible regulation of nitrogenase activity that was correlated with the reversible ADP-ribosylation of dinitrogenase reductase in vivo. Thus, the expression of draT and draG genes in K. pneumoniae is necessary and sufficient to support NH4(+)-switch-off/on, and ADP-ribosylation serves as a reversible regulatory mechanism for c...Continue Reading

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Citations

Sep 1, 1994·Molecular and Cellular Biochemistry·P W Ludden
Sep 1, 1994·Molecular and Cellular Biochemistry·N J Oppenheimer
Jan 9, 1992·Biochimica Et Biophysica Acta·P C Hallenbeck
Feb 1, 1995·Trends in Cell Biology·G de MurciaS Shall
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·T ArcondéguyM Merrick
Oct 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christoph Mueller-DieckmannFriedrich Koch-Nolte
Aug 13, 2014·Journal of Experimental Botany·T L G CarvalhoA S Hemerly
Nov 4, 2017·Critical Reviews in Biochemistry and Molecular Biology·Kerryanne CrawfordIvan Ahel
Dec 10, 1990·FEBS Letters·G H Leno, B E Ledford
Nov 21, 1992·BMJ : British Medical Journal·L J Donaldson
Jul 15, 1997·FEMS Microbiology Letters·Y ZhangG P Roberts
Dec 1, 1995·Microbiological Reviews·M J Merrick, R A Edwards

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