Reversible assembly of the ATP-binding cassette transporter Mdl1 with the F1F0-ATP synthase in mitochondria

The Journal of Biological Chemistry
Dominik Galluhn, Thomas Langer

Abstract

The half-ABC transporter Mdl1 is localized in the inner membrane of mitochondria and mediates the export of peptides generated upon proteolysis of mitochondrial proteins. The physiological role of the peptides released from mitochondria is currently not understood. Here, we have analyzed the oligomeric state of Mdl1 in the inner membrane and demonstrate nucleotide-dependent binding to the F(1)F(0)-ATP synthase. Mdl1 forms homo-oligomeric, presumably dimeric complexes in the presence of ATP, but was found in association with the F(1)F(0)-ATP synthase at low ATP levels. Mdl1 binds membrane-embedded parts of the ATP synthase complex after the assembly of the F(1) and F(0) moieties. Although independent of Mdl1 activity, complex formation is impaired upon inhibition of the F(1)F(0)-ATP synthase with oligomycin or N,N'-dicyclohexylcarbodiimide. These results are consistent with an activation of Mdl1 upon dissociation from the ATP synthase and suggest a link of peptide export from mitochondria to the activity of the F(1)F(0)-ATP synthase and the cellular energy metabolism.

References

Jan 1, 1991·Methods in Enzymology·T D FoxM C Costanzo
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M Desautels, A L Goldberg
Jun 5, 1999·The EMBO Journal·A KiharaK Ito
May 31, 2001·Methods in Cell Biology·H Schägger
Feb 2, 2002·The EMBO Journal·Patrick PaumardJean Velours
Mar 15, 2002·Trends in Biochemical Sciences·Daniel WipfWolf B Frommer
Aug 23, 2002·Biochimica Et Biophysica Acta·Isabel Arnold, Thomas Langer
Aug 29, 2002·The EMBO Journal·Quan ZhaoNicholas J Hoogenraad
Dec 31, 2002·Current Opinion in Structural Biology·Lutz Schmitt, Robert Tampé
Jul 24, 2003·Journal of Molecular Biology·Maja ChloupkováDavid M Koeller
Oct 11, 2003·Molecular Cell·Zhengchang LiuRonald A Butow
Mar 5, 2004·The Journal of Biological Chemistry·Alexander TzagoloffJohannes M Herrmann

❮ Previous
Next ❯

Citations

May 20, 2006·Pflügers Archiv : European journal of physiology·Meike Herget, Robert Tampé
Apr 23, 2013·Interdisciplinary Sciences, Computational Life Sciences·Md Rezaul IslamMd Ohedul Islam
Apr 29, 2010·The Journal of Biological Chemistry·Prakash G ChandakDagmar Kratky
Jan 13, 2006·FEBS Letters·Helmut Jungwirth, Karl Kuchler
Jun 15, 2007·Critical Reviews in Biochemistry and Molecular Biology·Mirko Koppen, Thomas Langer
Jul 28, 2007·Translational Research : the Journal of Laboratory and Clinical Medicine·Michael A Burke, Hossein Ardehali
Mar 3, 2009·Biochimica Et Biophysica Acta·Ariane ZutzRobert Tampé
Jun 28, 2015·European Journal of Cell Biology·Ulrich MühlenhoffRoland Lill
Jun 18, 2011·Biochimica Et Biophysica Acta·Holger Seelert, Norbert A Dencher
Oct 18, 2011·Biochimica Et Biophysica Acta·Florian GerdesThomas Langer
Dec 19, 2017·Frontiers in Microbiology·Andreas MosbachGabriel Scalliet
Nov 24, 2004·The Journal of Biological Chemistry·Steffen AugustinThomas Langer
May 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martha M MonickGary W Hunninghake

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.