Reversible dioxygen binding in solvent-free liquid myoglobin.

Nature Chemistry
Adam W PerrimanStephen Mann

Abstract

The ensemble of forces that stabilize protein structure and facilitate biological function are intimately linked with the ubiquitous aqueous environment of living systems. As a consequence, biomolecular activity is highly sensitive to the interplay of solvent-protein interactions, and deviation from the native conditions, for example by exposure to increased thermal energy or severe dehydration, results in denaturation and subsequent loss of function. Although certain enzymes can be extracted into non-aqueous solvents without significant loss of activity, there are no known examples of solvent-less (molten) liquids of functional metalloproteins. Here we describe the synthesis and properties of room-temperature solvent-free myoglobin liquids with near-native structure and reversible dioxygen binding ability equivalent to the haem protein under physiological conditions. The realization of room-temperature solvent-free myoglobin liquids with retained function presents novel challenges to existing theories on the role of solvent molecules in structural biology, and should offer new opportunities in protein-based nanoscience and bionanotechnology.

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Citations

Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Atim A EnyenihiRoman A Zubarev
Jul 14, 2011·ACS Nano·Adam W Perriman, Stephen Mann
Apr 4, 2012·Annual Review of Chemical and Biomolecular Engineering·Michael J LiszkaDouglas S Clark
Oct 7, 2014·Nature Communications·Alex P S BroganStephen Mann
Mar 16, 2016·Journal of the American Chemical Society·Alex P S Brogan, Jason P Hallett
Jun 1, 2016·Biochimica Et Biophysica Acta·Lada Biedermannová, Bohdan Schneider
Nov 20, 2013·Journal of the American Chemical Society·Kamendra P SharmaDavid J Fermin
Sep 10, 2014·The Journal of Physical Chemistry. B·Kamendra P SharmaStephen Mann
Mar 1, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Hongguang LiTakashi Nakanishi
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Jun 25, 2013·The Journal of Physical Chemistry. B·Alex P S BroganStephen Mann
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