PMID: 1201465Nov 24, 1975Paper

Reversible extinction of the morphogen in bone matrix by reduction and oxidation of disulfide bonds

Calcified Tissue Research
M R UristC N Conteas

Abstract

Beta-Mercaptoethanol (beta-ME) or dithiothreitol (DTT) reduction extinguishes the capacity of bone matrix gelatin to produce new bone following implantation in a muscle pouch. If the reducing solution is used in concentrations of 50 mmoles/l or less, the extinction can be partially reversed by bubbling of oxygen through the solution for one hour. Sulfhydryl group blocking reagents prevent reoxidation of reduced bone gelatin, and restoration of the bone morphogenetic property (BMP). Unspecific borohydride reduction at 37 degrees destroys bone yield irreversibly, but at 2 degrees reduction of free aldehyde groups in bone gelatin does not prevent beta-ME and DTT reversible extinction. These observations are interpreted to suggest that the disulphide linkage may be an essential part of the biologically active conformation of either a non-collagenous bone morphogenetic polypeptide firmly bound to collagen or a collagen by-product entrapped within a water insoluble gel matrix.

References

Jan 1, 1973·Journal of Theoretical Biology·M R Urist, H Iwata
Feb 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M R UristP L Ceccotti
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M R UristC Chien
Jan 1, 1974·Calcified Tissue Research·M R UristH Iwata
Nov 1, 1971·Journal of Dental Research·M R Urist, B S Strates
Jun 29, 1971·Biochimica Et Biophysica Acta·A Z Budzynski, G E Means

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Citations

Jan 1, 1984·Calcified Tissue International·S MohanD Baylink
Feb 1, 1997·Mund-, Kiefer- und Gesichtschirurgie : MKG·N R Kübler
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M R UristA Lietze
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M R UristR J DeLange
Jan 19, 2013·The International Journal of Neuroscience·Ji-Hong XuZhi-Guo Yuan
Jan 1, 1977·Preparative Biochemistry·A J Mikulski, M R Urist
Dec 14, 2002·Genesis : the Journal of Genetics and Development·Guang-Quan Zhao
Mar 1, 1977·Journal of Dental Research·W T ButlerS Uyeno
Apr 21, 2001·The Journal of Craniofacial Surgery·H G MoghadamC M Clokie
Mar 4, 2000·Plastic and Reconstructive Surgery·C M Clokie, M R Urist
Oct 18, 2006·Antiviral Chemistry & Chemotherapy·Mimi RemichkovaAngel S Galabov
May 13, 1983·Science·M R UristG A Finerman
Dec 1, 1993·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·N KüblerW Sebald

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