Reversible (G0) and nonreadily reversible (Q) noncycling cells in human peripheral blood. Immunological, structural, and biological characterization

Cell Biophysics
S AbrahamC A Nicolini

Abstract

PHA-stimulated human lymphocytes (normal-resting-proliferating) at 0, 24, 48, 72, and 144 h were studied with Acridine Orange (AO) staining. By viable cell sorting, by subsequent subculturing, and by the use of biochemical, biophysical, and immunological assays, not only have the G0 resting and G1 (cycling) cell cycle phases been objectively characterized, but a separate subpopulation of quiescent cells that are functionally viable and deeply committed to nonproliferation, the Q cells, has been identified. Multiparameter cytofluorimetric analysis, methyl14C-thymidine incorporation, automated image analysis, and mitogen stimulation studies have shown that the "Q" cell, compared to the "G0" resting but easily recruitable cell, exhibits quite lower red and green AO emission, possesses 2c to 4c DNA content (rather than only 2c), has a higher average optical density, and is either nonrecruitable or recruitable-with-difficulty in PHA-stimulated lymphocyte cultures.

References

Apr 1, 1977·Experimental Cell Research·C NicoliniJ Fried
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C NicoliniS Abraham
Feb 23, 1976·Biochemical and Biophysical Research Communications·S P HawkesM J Bissell

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Citations

Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Claudio Nicolini
Jun 24, 2006·Journal of Cellular Biochemistry·Luca Giacomelli, Claudio Nicolini
Nov 30, 2005·Journal of Cellular Biochemistry·V SivozhelezovC Nicolini
Nov 30, 2005·Journal of Cellular Biochemistry·Claudio NicoliniLuca Giacomelli
Aug 25, 2007·Nanomedicine·Claudio Nicolini
Jan 1, 1983·Pharmacology & Therapeutics·J W Gray
Dec 22, 1983·Biochimica Et Biophysica Acta·D A PrenticeL R Gurley

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