Reversible immobilization of uricase on conductive polyaniline brushes grafted on polyacrylonitrile film

Bioprocess and Biosystems Engineering
Gulay BayramogluM Yakup Arıca

Abstract

Polyacrylonitrile film (PAN) surfaces were modified with chemical polymerization of conductive polyaniline (PANI) in the presence of potassium dichromate as an oxidizing agent. The conductive films were used for immobilization of uricase. The surface resistance of the conductive film in this work was found to be 0.97 kΩ/cm. The maximum amount of immobilized enzyme on conductive film containing 2.4% PANI was about 216 μg/cm(2). The optimum pH for free and immobilized enzymes was observed at 7.0 and 7.5, respectively. The K (m) values for free and immobilized uricase were found to be 94 and 138 μM, respectively. V (max) values were calculated as 1.87 and 1.63 U/mg protein for the free and immobilized enzymes, respectively. Immobilized uricase exhibited ~68% of its original activity even after 2 months of storage at 4 °C while the free enzyme lost its initial activity within 4 weeks.

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Citations

Dec 3, 2013·Analytical Biochemistry·V Cengiz OzalpM Yakup Arica
Mar 28, 2012·Bioprocess and Biosystems Engineering·Gulay BayramogluM Yakup Arica
Apr 30, 2013·Bioprocess and Biosystems Engineering·Kerem UzunAbdülhadi Baykal
Mar 16, 2012·Applied Microbiology and Biotechnology·Gulay BayramogluM Yakup Arica
Dec 10, 2014·Applied Biochemistry and Biotechnology·Prashanth RamachandranUma Maheswari Krishnan
Dec 15, 2012·Biochemistry·Michael S CavesRobert B Freedman

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