PMID: 6108209Sep 1, 1980Paper

Reversible phosphorylation and inactivation of acetyl-CoA carboxylase from lactating rat mammary gland by cyclic AMP-dependent protein kinase

European Journal of Biochemistry
D G Hardie, P S Guy

Abstract

Acetyl-CoA carboxylase has been purified from lactating rat mammary gland using a combination of ammonium sulphate and poly(ethyleneglycol) precipitations. The enzyme was purified from 35--70-fold with a yield of over 50%, the exact figures being difficult to estimate because of activation of the enzyme that occurs during the preparation. The preparation was homogeneous by the criterion of polyacrylamide gel electrophoresis in sodium dodecyl sulphate and had a single subunit of molecular weight 240,000, containing 1.02 +/- 0.04 molecules of biotin and 3.1 +/- 1.7 molecules of alkali-labile phosphate per subunit. The purified enzyme was phosphorylated and inactivated rapidly when incubated in the presence of [gamma 32P]ATP and magnesium ions with the purified catalytic subunit of cyclic-AMP-dependent protein kinase from rabbit skeletal muscle. Both phosphorylation and inactivation are blocked by the heat-stable protein inhibitor of cyclic-AMP-dependent protein kinase, and can be reversed by incubation with purified protein phosphatase-1 from rabbit skeletal muscle. The inactivation by the protein kinase and reactivation by the protein phosphatase correlate with the near-stoichiometric phosphorylation and dephosphorylation of sit...Continue Reading

References

Nov 1, 1976·The Biochemical Journal·J H WalkerR J Mayer
Feb 15, 1977·The Biochemical Journal·P CohenJ F Antoniw
Jul 1, 1978·Hoppe-Seyler's Zeitschrift für physiologische Chemie·E A SiessO H Wieland
Sep 1, 1976·European Journal of Biochemistry·H G NimmoP Cohen
Mar 1, 1973·The Biochemical Journal·A P Halestrap, R M Denton
Nov 1, 1973·Biochemical and Biophysical Research Communications·G J Klain, P C Weiser
Oct 1, 1974·Archives of Biochemistry and Biophysics·C A Carlson, K H Kim
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J A BeavoE G Krebs
Oct 1, 1973·Biochemical and Biophysical Research Communications·K H LeeK H Kim

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Citations

Jan 1, 1995·Archives of Dermatological Research·C M HurtK R Feingold
Apr 5, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Min Seon Kim, Ki Up Lee
Sep 1, 1983·Archives of Biochemistry and Biophysics·B A Lent, K H Kim
Jan 1, 1983·The International Journal of Biochemistry·P M Gillevet, K Dakashinamurti
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M N Abdel-Halim, S I Farah
Sep 2, 1999·Advances in Enzyme Regulation·M R Munday, C J Hemingway
Jun 5, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jorge DiasSadasivam Kaushik
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J SommercornE G Krebs
Dec 3, 2014·Molecular and Cellular Endocrinology·Miguel López, Carlos Diéguez
Jun 1, 1983·The Proceedings of the Nutrition Society·R G Vernon, D J Flint
Mar 8, 2005·Biochimica Et Biophysica Acta·Michael C BarberMaureen T Travers
Dec 13, 2002·Emergency Medicine Clinics of North America·Gregory Luke Larkin, Raymond Logan Fowler
Oct 1, 1996·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine
Dec 1, 1983·Archives of Biochemistry and Biophysics·J P TipperL A Witters
Dec 16, 1983·Biochemical and Biophysical Research Communications·S Ramakrishna, W B Benjamin
Sep 1, 1983·European Journal of Biochemistry·K Wada, T Tanabe
Nov 1, 1993·European Journal of Biochemistry·A BaquetL Hue

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