Reversible photo-regulation on the folding/unfolding of telomere G-quadruplexes with solid-state nanopores.

The Analyst
Jing TangLiyuan Liang

Abstract

The formation of G-quadruplexes (G4) in human telomere and other important biological regions inhibits the replication and transcription of DNA, thereby influencing further cell proliferation. The investigation of G4 formation and unfolding is vital for understanding their modulation in biological processes and life science. Photo regulation is a facile and sensitive approach for monitoring the structures of biomacromolecules and material surface properties. The nanopore-based technique is also prevalent for label-free single-molecule characterization with high accuracy. This study provides a combination of solid-state nanopore technology with light-switch as a platform for the modulation of human telomere G4 formation and splitting under switchable light exposure. The introduction of molecular switch, namely azobenzene moiety at different positions of the DNA sequence influences the formation and stability of G4. Three azobenzenes immobilized on each of the G-quartet plane (hTelo-3azo-p) or four azobenzenes on the same plane (hTelo-4azo-4p) of the human telomere G4 sequence realized the reversible control of G4 folding/unfolding at the temporal scale upon photo regulation, and the formation and splitting of G4 with hTelo-4azo-...Continue Reading

References

Feb 23, 2010·Nanotechnology·Michiel van den HoutNynke H Dekker
Jun 24, 2010·Angewandte Chemie·Xiaolin WangXiang Zhou
Oct 26, 2010·Nature Nanotechnology·Meni WanunuMarija Drndić
Jul 7, 2011·Nanotechnology·Stefan W KowalczykCees Dekker
Apr 11, 2012·Methods : a Companion to Methods in Enzymology·Jiwook Shim, Li-Qun Gu
Sep 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Na AnCynthia J Burrows
Dec 19, 2014·Angewandte Chemie·Fuan WangItamar Willner
Sep 10, 2015·Nucleic Acids Research·Daniela Rhodes, Hans J Lipps
Feb 4, 2016·Journal of the American Chemical Society·Eric LargyJean-Louis Mergny
Sep 27, 2016·Frontiers in Chemistry·Debmalya BhattacharyyaSoumitra Basu
Jan 28, 2017·Chemical Society Reviews·Anouk S LubbeBen L Feringa
Mar 28, 2017·Nature Nanotechnology·Prakash ShresthaHanbin Mao
Apr 19, 2017·Bioorganic & Medicinal Chemistry Letters·Han Na JooYoung Jun Seo
Aug 11, 2017·Chemical Communications : Chem Comm·Fu-Na MengYi-Tao Long
Nov 15, 2017·Nature Communications·Xinyue ZhangLi-Qun Gu
Sep 6, 2018·Science Advances·Scarlett A DvorkinMateus Webba da Silva
Jan 4, 2019·Chemical Reviews·Jean-Louis Mergny, Dipankar Sen
Apr 27, 2019·Journal of Medicinal Chemistry·Zhen YuJ A Cowan
Jun 7, 2019·Nature Protocols·Rui GaoYi-Tao Long
Jul 10, 2019·Nucleic Acids Research·Anoja MegalathanSoma Dhakal
Oct 3, 2019·Nano Letters·Filip BoškovićUlrich F Keyser
Oct 28, 2019·Biomaterials Science·Zhongtao Wu, Lei Zhang
Dec 15, 2019·Nature Protocols·Matthew WaughVincent Tabard-Cossa
Mar 18, 2020·Analytical Chemistry·Si-Min LuYi-Tao Long
Apr 10, 2020·Analytical Chemistry·Liangliang ZhangFengyu Li

❮ Previous
Next ❯

Citations

Aug 28, 2021·Nanomaterials·Xiaoqing ZengDeqiang Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
Fluorescence

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.