Reversible redox modifications in the microglial proteome challenged by beta amyloid

Molecular BioSystems
Virginia CorreaniM Eugenia Schininà

Abstract

Microglia are resident macrophages in the central nervous system, whose participation against exogenous injuries and infections is mainly marked by an immediate release of inflammatory cytokines along with a toxic efflux of superoxide radicals. Indeed, many lines of evidence indicate that persistent activation of these cells turns their neuroprotective phenotype into a neurotoxic one, which contributes to destroy neuronal activity and induces neuronal loss in several neurodegeneration processes, such as Alzheimer's disease. In this study we attempted to fill-in the gap in our knowledge about redox regulation of amyloid activated microglia. With this aim, we carried out a robust and comprehensive characterization of the reversibly redox modified proteome both at the level of resting and amyloid-activated BV2 cells, an immortalised cell line of murine microglia. The approach we used combined the selective enrichment of reversible redox modified proteins through a biotin bait with nanoscale liquid chromatography tandem mass spectrometry of their proteolytic peptides. By this reliable approach, we identified 60 proteins changing the redox status of their selective cysteine residues upon treatment with the amyloidogenic Aβ25-35 pept...Continue Reading

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Citations

Sep 7, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Selvaraj MiltonprabuSeyed Mohammad Nabavi
Sep 27, 2016·Proteomics. Clinical Applications·Liqing Gu, Renã A S Robinson
Apr 21, 2020·Biochemical Society Transactions·Loes van Dam, Tobias B Dansen
Aug 18, 2017·Proteomics·Virginia CorreaniM Eugenia Schininà
Nov 12, 2018·Biochemical Pharmacology·Virginia CorreaniMaria d'Erme
May 21, 2021·Molecular Neurobiology·Jiangfeng LiaoJing Zhang

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