PMID: 6537990Jan 1, 1984Paper

Reversible shape change of Triton-treated erythrocyte ghosts induced by Ca2+ and Mg-ATP

Nature
Y JinbuM NAKAO

Abstract

The shape of the human erythrocyte depends on intracellular ATP content and echinocytic erythrocyte ghosts obtained in the presence of Mg-ATP acquire a diskocytic shape after incubation at 37 degrees C; however, agreement is lacking about the molecular basis of the shape changes. The suggestion that phosphorylation of cytoskeletal structures underlying the membrane is involved has been disputed and alternative explanations based on lipid bilayer theory and metabolism of phospholipid have been proposed. Recently, we re-examined the effect of ATP on the shape of ghosts and found that it consists of two distinct steps. We have, therefore, now examined the effect of physiological concentrations of Ca2+ and ATP on the cytoskeleton of the erythrocyte membrane directly using Triton-treated ghosts which had lost this permeability barrier. Our findings suggest that a noncovalent effect of ATP on the cytoskeleton is a prerequisite for shape change.

References

Dec 1, 1979·The Journal of Cell Biology·S B Hellewell, D L Taylor
Jun 1, 1977·The Journal of Cell Biology·W Birchmeier, S J Singer
Jul 1, 1976·The Journal of Cell Biology·M P SheetzS J Singer
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M P Sheetz, S J Singer
Apr 29, 1983·Biochemical and Biophysical Research Communications·Y JinbuS Sato
Mar 1, 1983·The Journal of Cell Biology·G A NelsonM J Karnovsky
Aug 1, 1980·The Journal of Cell Biology·R M JohnsonD B Meyer
Jan 1, 1981·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·G FairbanksJ E Dino
Aug 14, 1980·Biochemical and Biophysical Research Communications·E E Quist, K L Reece
Jun 1, 1961·Journal of Biochemistry·M NAKAOH YOSHIKAWA

❮ Previous
Next ❯

Citations

Aug 1, 1991·Journal of Cellular Biochemistry·Y H XuB D Roufogalis
Feb 15, 2002·Current Opinion in Hematology·Makoto Nakao
Jan 25, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nadezhda N BarvitenkoRoy E Weber
May 16, 1990·Biochemical and Biophysical Research Communications·M NakaoK Wake
Apr 1, 1986·Archives of Biochemistry and Biophysics·D Maretzki, H U Lutz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.