Reversible stalling of transcription elongation complexes by high pressure

Biophysical Journal
L Erijman, R M Clegg

Abstract

We have investigated the effect of high hydrostatic pressure on the stability of RNA polymerase molecules during transcription. RNA polymerase molecules participating in stalled or active ternary transcribing complexes do not dissociate from the template DNA and nascent RNA at pressures up to 180 MPa. A lower limit for the free energy of stabilization of an elongating ternary complex relative to the quaternary structure of the free RNAP molecules is estimated to be 20 kcal/mol. The rate of elongation decreases at high pressure; transcription completely halts at sufficiently high pressure. The overall rate of elongation has an apparent activation volume (DeltaVdouble dagger) of 55-65 ml . mol-1 (at 35 degrees C). The pressure-stalled transcripts are stable and resume elongation at the prepressure rate upon decompression. The efficiency of termination decreases at the rho-independent terminator tR2 after the transcription reaction has been exposed to high pressure. This suggests that high pressure modifies the ternary complex such that termination is affected in a manner different from that of elongation. The solvent and temperature dependence of the pressure-induced inhibition show evidence for major conformational changes in th...Continue Reading

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Citations

May 2, 2002·Biochimica Et Biophysica Acta·Jack A Kornblatt, M Judith Kornblatt
May 2, 2002·Biochimica Et Biophysica Acta·Robert B Macgregor
Aug 21, 2012·Environmental Science & Technology·Benjamin LemaireJean François Rees
May 7, 2004·Applied and Environmental Microbiology·Abram AertsenChris W Michiels
May 5, 2005·Applied and Environmental Microbiology·Abram AertsenChris W Michiels
Sep 3, 2004·Journal of Bacteriology·Abram AertsenChris W Michiels
Feb 19, 2016·The Journal of Physical Chemistry. B·Gusztáv SchayJudit Fidy
Dec 7, 2007·Biophysical Journal·Mikael EsmannDerek Marsh
Nov 30, 2005·Molecular Microbiology·Abram Aertsen, Chris W Michiels
Jan 30, 2014·Journal of the Science of Food and Agriculture·Ling-Lin FuJunda Lin
Nov 6, 2009·Current Opinion in Structural Biology·Vladimir Svetlov, Evgeny Nudler
Jul 17, 2019·Journal of Molecular Biology·Jin Young KangSeth A Darst
Nov 21, 2007·Biomacromolecules·David PastréOlivier Piétrement
Nov 5, 1999·Trends in Microbiology·F AbeK Horikoshi
Aug 5, 2021·Annual Review of Microbiology·Robert Landick

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