Reversing Antimicrobial Resistance in Multidrug-Resistant Klebsiella pneumoniae of Clinical Origin Using 1-(1-Naphthylmethyl)-Piperazine

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
João AnesSeámus Fanning

Abstract

Eleven clinical Klebsiella pneumoniae fluoroquinolone-resistant isolates were tested to access the potential of adjuvant therapies to reduce antimicrobial resistance using fixed concentrations of the chemosensitizers chlorpromazine (CPZ), thioridazine (TZ), phenylalanine-arginine-β-naphthylamide (PAβN), and 1-(1-naphthylmethyl)-piperazine-(NMP) with varying concentrations of antimicrobial agents nalidixic acid (NAL), ciprofloxacin (CIP), moxifloxacin (MXF), tetracycline (TET), and chloramphenicol (CHL). Ethidium bromide dye was used together with the chemosensitizers to investigate permeabilization effects. NMP was assessed for its capacity to reduce the mass of biofilm alone and in combination with CIP and MXF. Of the selected chemosensitizers, NMP exhibited the greatest capacity to reverse resistance and inhibit efflux, based on the concentrations tested. Susceptibility to antimicrobial agents including (fluoro)quinolones, TET, and CHL were found to be increased in the presence of NMP, in a concentration-dependent manner. PAβN also demonstrated similar effects when combined with the chemosensitizers tested. In the case of half of the isolates studied, NMP alone reduced preformed biofilm biomass. Combinations of latter along w...Continue Reading

References

Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A A NeyfakhL B Chen
Nov 1, 1996·Antimicrobial Agents and Chemotherapy·P S Stewart
Feb 13, 2001·Trends in Microbiology·T F Mah, G A O'Toole
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·I Chopra, M Roberts
Jan 21, 2004·Molecular Microbiology·Christophe BeloinJean-Marc Ghigo
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Jürgen A Bohnert, Winfried V Kern
Oct 7, 2008·Applied and Environmental Microbiology·Malin KvistPer Klemm
Oct 20, 2009·Journal of Biological Engineering·Laura PaixãoMiguel Viveiros
Dec 17, 2009·International Journal of Antimicrobial Agents·Asish DasguptaLeonard Amaral
Feb 2, 2011·Antimicrobial Agents and Chemotherapy·E C SpindlerR T Gill
Feb 24, 2011·Expert Reviews in Molecular Medicine·Lindsay Kalan, Gerard D Wright
May 10, 2011·FEBS Letters·Jean-Michel BollaJean-Marie Pagès
Dec 14, 2012·Annals of Clinical Microbiology and Antimicrobials·Grace C Lee, David S Burgess
Jan 18, 2013·Journal of Global Infectious Diseases·Monil SinghaiRajeev Goyal
Mar 16, 2013·The Journal of Antimicrobial Chemotherapy·A J LawlerL J V Piddock
Apr 6, 2013·The Open Microbiology Journal·Jürgen A BohnertWinfried V Kern
Nov 20, 2013·Antimicrobial Agents and Chemotherapy·Timothy J OppermanTerry L Bowlin
Dec 18, 2013·Nature Reviews. Microbiology·Daniel N Wilson
May 20, 2014·The Journal of Infectious Diseases·David LebeauxJean-Marc Ghigo
May 21, 2014·Trends in Microbiology·Liam S RedgraveLaura J V Piddock
Aug 13, 2014·Antimicrobial Agents and Chemotherapy·Attilio V VargiuHiroshi Nikaido
Sep 7, 2014·The Journal of Antimicrobial Chemotherapy·Suzanne Bialek-DavenetMarie-Hélène Nicolas-Chanoine
Sep 17, 2014·MicrobiologyOpen·Thelma Ohene-AgyeiHenrietta Venter
Oct 4, 2014·Frontiers in Public Health·Carolyn Anne MichaelMaurizio Labbate
Oct 12, 2014·Trends in Microbiology·Liang ChenBarry N Kreiswirth
Dec 2, 2014·Pathogens·Claudia VuottoPietro E Varaldo
May 23, 2015·Frontiers in Microbiology·Timothy J Opperman, Son T Nguyen
Jun 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kathryn E HoltNicholas R Thomson

❮ Previous
Next ❯

Software Mentioned

Prism
Excel

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here