Reversing methanogenesis to capture methane for liquid biofuel precursors

Microbial Cell Factories
Valerie W C SooThomas K Wood

Abstract

Energy from remote methane reserves is transformative; however, unintended release of this potent greenhouse gas makes it imperative to convert methane efficiently into more readily transported biofuels. No pure microbial culture that grows on methane anaerobically has been isolated, despite that methane capture through anaerobic processes is more efficient than aerobic ones. Here we engineered the archaeal methanogen Methanosarcina acetivorans to grow anaerobically on methane as a pure culture and to convert methane into the biofuel precursor acetate. To capture methane, we cloned the enzyme methyl-coenzyme M reductase (Mcr) from an unculturable organism, anaerobic methanotrophic archaeal population 1 (ANME-1) from a Black Sea mat, into M. acetivorans to effectively run methanogenesis in reverse. Starting with low-density inocula, M. acetivorans cells producing ANME-1 Mcr consumed up to 9 ± 1 % of methane (corresponding to 109 ± 12 µmol of methane) after 6 weeks of anaerobic growth on methane and utilized 10 mM FeCl3 as an electron acceptor. Accordingly, increases in cell density and total protein were observed as cells grew on methane in a biofilm on solid FeCl3. When incubated on methane for 5 days, high-densities of ANME-1 ...Continue Reading

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Jan 19, 2016·Microbial Cell Factories·Hadi Nazem-BokaeeCostas D Maranas
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Datasets Mentioned

BETA
FP565147.1
GSE66445

Methods Mentioned

BETA
PCR
nuclear magnetic resonance
NMR
Assay
cDNA library

Software Mentioned

Cufflink
hsqcetgpsisp
Picard
STAR
Unified Genotyper
vcftools
Genome Analysis ToolKit package
SortSam
Cuffdiff
IndelRealigner

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