Reversing the resistance phenotype of the Biomphalaria glabrata snail host Schistosoma mansoni infection by temperature modulation.

PLoS Pathogens
Wannaporn Ittiprasert, M Knight

Abstract

Biomphalaria glabrata snails that display either resistant or susceptible phenotypes to the parasitic trematode, Schistosoma mansoni provide an invaluable resource towards elucidating the molecular basis of the snail-host/schistosome relationship. Previously, we showed that induction of stress genes either after heat-shock or parasite infection was a major feature distinguishing juvenile susceptible snails from their resistant counterparts. In order to examine this apparent association between heat stress and snail susceptibility, we investigated the effect of temperature modulation in the resistant snail stock, BS-90. Here, we show that, incubated for up to 4 hrs at 32°C prior to infection, these resistant snails became susceptible to infection, i.e. shedding cercariae at 5 weeks post exposure (PE) while unstressed resistant snails, as expected, remained resistant. This suggests that susceptibility to infection by this resistant snail phenotype is temperature-sensitive (ts). Additionally, resistant snails treated with the Hsp 90 specific inhibitor, geldanamycin (GA) after heat stress, were no longer susceptible to infection, retaining their resistant phenotype. Consistently, susceptible snail phenotypes treated with 100 mM GA ...Continue Reading

References

Jan 1, 1988·Annual Review of Genetics·S Lindquist, E A Craig
Sep 1, 1986·Revista Do Instituto De Medicina Tropical De São Paulo·L C Dias, C E Olivier
Mar 1, 1995·Memórias do Instituto Oswaldo Cruz·A J SimpsonS D Pena
May 5, 1998·Memórias do Instituto Oswaldo Cruz·F A LewisC S Richards
Jan 28, 1999·Memórias do Instituto Oswaldo Cruz·D RollinsonL R Noble
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·M KnightF A Lewis
Jul 27, 2001·The Journal of Cell Biology·J C YoungF U Hartl
Oct 16, 2001·Cell Stress & Chaperones·R H KimuraC W Schmid
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 10, 2002·Memórias do Instituto Oswaldo Cruz·Herminia Y KanamuraRaymond T Damian
Dec 12, 2002·Cellular and Molecular Life Sciences : CMLS·D Picard
Feb 14, 2003·The Journal of Biological Chemistry·Gowrishankar BanumathyUtpal Tatu
Mar 5, 2003·Molecular and Biochemical Parasitology·Nithya RaghavanMatty Knight
Nov 1, 1954·Experimental Parasitology·M A STIREWALT
May 1, 1957·Transactions of the Royal Society of Tropical Medicine and Hygiene·E PAULINI, J PELLEGRINO
Sep 13, 2005·Journal of Helminthology·A Théron, C Coustau
Oct 1, 2005·International Journal for Parasitology·Judith E Humphries, Timothy P Yoshino
Feb 14, 2006·AIDS·Eyrun F KjetlandSvein Gunnar Gundersen
Apr 25, 2006·Biochemical and Biophysical Research Communications·Yuo-Sheng ChangYiu-Kay Lai
Oct 13, 2006·Trends in Parasitology·Charles H KingJoseph Hamburger
Jun 20, 2008·American Journal of Physiology. Renal Physiology·Ewen M HarrisonStephen J Wigmore
Jun 26, 2008·Novartis Foundation Symposium·Martha TriantafilouKathy Triantafilou
Aug 6, 2008·Annual Review of Genetics·Arthur BeauregardMarlene Belfort
Sep 26, 2008·Parasite : Journal De La Société Française De Parasitologie·A BouchutG Mitta
May 15, 2009·Molecular and Biochemical Parasitology·Matty KnightChristopher J Bayne
Jul 28, 2009·Parasitology·David RollinsonJ Russell Stothard

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Citations

Nov 8, 2013·Canadian Journal of Zoology·T P YoshinoC J Bayne
Mar 15, 2015·Fish & Shellfish Immunology·Michelle A GordyPatrick C Hanington
May 20, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·A TheronG Mitta
Apr 17, 2016·Parasitology Research·Pattarakul PakchotanonPoom Adisakwattana
Aug 8, 2014·Frontiers in Genetics·Matty KnightJoanna M Bridger
Sep 12, 2014·PLoS Neglected Tropical Diseases·Halime D Arican-GoktasMatty Knight
Mar 17, 2015·PLoS Genetics·Jacob A TennessenMichael S Blouin
Sep 10, 2016·PLoS Neglected Tropical Diseases·Kenji Ishida, Emmitt R Jolly
Aug 10, 2018·PLoS Neglected Tropical Diseases·Joanna M BridgerMatty Knight
Oct 3, 2018·Tropical Medicine and Infectious Disease·Damilare O Famakinde

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Datasets Mentioned

BETA
AA547771

Methods Mentioned

BETA
genotyping
dissecting
PCR
electrophoresis

Software Mentioned

Primer
Quantity One
- Blast

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