Oct 2, 2020

Combinatorial patterns of graded RhoA activation and uniform F-actin depletion promote tissue curvature

BioRxiv : the Preprint Server for Biology
M. Denk-LobnigAdam C Martin


During development, gene expression regulates cell mechanics and shape to sculpt tissues. Epithelial folding proceeds through distinct cell shape changes that occur in different regions of a tissue. Here, using quantitative imaging in Drosophila melanogaster, we investigate how patterned cell shape changes promote tissue bending during early embryogenesis. We find that the transcription factors Twist and Snail combinatorially regulate a unique multicellular pattern of junctional F-actin density, which corresponds to whether cells apically constrict, stretch, or maintain their shape. Part of this pattern is a gradient in junctional F-actin and apical myosin-2, and the width of this gradient regulates tissue curvature. The actomyosin gradient results from a gradient in RhoA activation that is refined by a balance between RhoGEF2 and the RhoGAP C-GAP. Thus, cell behavior in the ventral furrow is choreographed by the interplay of distinct gene expression patterns and this coordination regulates tissue shape.

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Congenital Hypoplasia of Adrenal Gland
Fish <Chondrichthyes>
World Health Organization
JAKMIP1 protein, human
Swordfish allergenic extract

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Nature Structural Biology
E H EgelmanA McGough
Journal of Molecular Biology
F D Carlson, A B Fraser
Journal of Muscle Research and Cell Motility
E H Egelman
The Journal of Cell Biology
Hélène A Benink, William M Bement
© 2020 Meta ULC. All rights reserved