Oct 2, 2020

Combinatorial patterns of graded RhoA activation and uniform F-actin depletion promote tissue curvature

BioRxiv : the Preprint Server for Biology
M. Denk-LobnigAdam C Martin

Abstract

During development, gene expression regulates cell mechanics and shape to sculpt tissues. Epithelial folding proceeds through distinct cell shape changes that occur in different regions of a tissue. Here, using quantitative imaging in Drosophila melanogaster, we investigate how patterned cell shape changes promote tissue bending during early embryogenesis. We find that the transcription factors Twist and Snail combinatorially regulate a unique multicellular pattern of junctional F-actin density, which corresponds to whether cells apically constrict, stretch, or maintain their shape. Part of this pattern is a gradient in junctional F-actin and apical myosin-2, and the width of this gradient regulates tissue curvature. The actomyosin gradient results from a gradient in RhoA activation that is refined by a balance between RhoGEF2 and the RhoGAP C-GAP. Thus, cell behavior in the ventral furrow is choreographed by the interplay of distinct gene expression patterns and this coordination regulates tissue shape.

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Mentioned in this Paper

Mercury
Study
JAKMIP1
Congenital Hypoplasia of Adrenal Gland
Environment
Fish <Chondrichthyes>
World Health Organization
JAKMIP1 protein, human
Swordfish allergenic extract
Zebrafish

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