PMID: 41222Oct 10, 1979

Review: ethidium fluorescence assays. Part 1. Physicochemical studies

Nucleic Acids Research
A R MorganD H Evans

Abstract

DNA and RNA can be assayed rapidly and very sensitively by exploiting the enhanced fluorescence of ethidium intercalated into duplex regions. By assaying at different pHs and introducing a heating/cooling cycle, a great many physicochemical aspects of DNA and RNA can be studied avoiding the use of radiolabels, and often giving information not otherwise readily obtainable. Studies are described on duplex DNA which involve measurement of extinction coefficients, cross-linking by chemicals, Cot curve analysis as well as estimation of drug-DNA binding constants. The assays can be adapted to investigate multi-stranded nucleic acid structures. The use of covalently closed circular DNA also allows rapid and extremely sensitive measurements of nicking caused by irradiation or drugs.

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Related Concepts

Novidium
Alkalescens-Dispar Group
Fluorescence Spectroscopy
Etiology
DNA Binding
Physicochemical Phenomena
DNA, Circular
15S RNA
Nucleic Acids
Bos indicus

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