Revised karyotyping and gene mapping of the Biomphalaria glabrata embryonic (Bge) cell line.

International Journal for Parasitology
Edwin C OdoemelamMatty Knight

Abstract

The fresh water snail Biomphalaria glabrata (2n=36) belongs to the taxonomic class Gastropoda (family Planorbidae) and is integral to the spread of the human parasitic disease schistosomiasis. The importance of this mollusc is such that it has been selected as a model molluscan organism for whole genome sequencing. In order to understand the structure and organisation of the B. glabrata's genome it is important that gene mapping studies are established. Thus, we have studied the genomes of two B. glabrata embryonic (Bge) cell line isolates 1 and 2 grown in separate laboratories, but both derived from Eder L. Hansen's original culture from the 1970s. This cell line continues to be an important tool and model system for schistosomiasis and B. glabrata. Using these cell line isolates, we have investigated the genome content and established a revised karyotype based on chromosome size and centromere position for these cells. Unlike the original karyotype (2n=36) established for the cell line, our investigations now show the existence of extensive aneuploidy in both cell line isolates to the extent that the total complement of chromosomes in both greatly exceeds the original cell line's diploid number of 36 chromosomes. The isolates...Continue Reading

References

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Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·M KnightF A Lewis
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Mar 5, 2003·Molecular and Biochemical Parasitology·Nithya RaghavanMatty Knight
Apr 15, 2004·Parasitology International·Philip T LoVerdeNajib El-Sayed
Apr 22, 2004·Trends in Parasitology·Najib M A El-SayedPhilip T LoVerde
Oct 1, 2005·International Journal for Parasitology·Judith E Humphries, Timothy P Yoshino
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Feb 25, 2006·Trends in Parasitology·Nithya Raghavan, Matty Knight
May 25, 2007·International Journal for Parasitology·Nithya RaghavanMatty Knight

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Citations

Nov 8, 2013·Canadian Journal of Zoology·T P YoshinoC J Bayne
Jul 19, 2011·PLoS Neglected Tropical Diseases·Matty KnightWannaporn Ittiprasert
May 15, 2009·Molecular and Biochemical Parasitology·Matty KnightChristopher J Bayne
Aug 8, 2014·Frontiers in Genetics·Matty KnightJoanna M Bridger
Sep 12, 2014·PLoS Neglected Tropical Diseases·Halime D Arican-GoktasMatty Knight
Aug 10, 2018·PLoS Neglected Tropical Diseases·Joanna M BridgerMatty Knight
May 17, 2017·Nature Communications·Coen M AdemaRichard K Wilson
Dec 20, 2019·PLoS Neglected Tropical Diseases·Theresa MaierJutta Reinhard-Rupp
Nov 25, 2019·Developmental and Comparative Immunology·Maria G CastilloCesar E Montelongo

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