Revised structure of the active form of human deoxyribonuclease IIalpha

Biochemical and Biophysical Research Communications
K S MacLeaA Eastman

Abstract

Deoxyribonuclease IIalpha (DNase IIalpha) is an acid endonuclease found in lysosomes, nuclei, and various secretions. Murine DNase IIalpha is required for digesting the DNA of apoptotic cells after phagocytosis and for correct development and viability. DNase IIalpha purified from porcine spleen was previously shown to contain three peptides, two of which were thiol crosslinked, all derived by processing of a single polypeptide. Commercial bovine protein is consistent with this structure. However, screening of 18 human cell lines failed to demonstrate this processing, rather a 45 kDa protein was consistently observed. Incubation of cells with the N-glycosylation inhibitor tunicamycin resulted in a 37 kDa protein, which is close to the predicted formula weight. The protein also contains at least one thiol crosslink. Similar results were obtained with overexpressed DNase IIalpha. These results suggest that active DNase IIalpha consists of one contiguous polypeptide. We suggest the previous structure reflects proteolysis during protein purification.

References

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Citations

Apr 19, 2008·DNA and Cell Biology·Ma-feng LiuMing-yuan Liu
Sep 10, 2005·Bioinformatics·Iwona A CymermanJanusz M Bujnicki
Mar 22, 2013·PloS One·Susumu OhkouchiYasuo Uchiyama
May 21, 2014·Preparative Biochemistry & Biotechnology·Zhiqiang ZhuBo Niu
Apr 4, 2017·Nucleic Acids Research·Armando Varela-RamirezRenato J Aguilera
Feb 28, 2006·Gene·Kyle S MacLea, Hans H Cheng
Jan 12, 2005·Clinical and Diagnostic Laboratory Immunology·Krisztina SallaiPeter Gergely
Jan 17, 2019·Journal, Genetic Engineering & Biotechnology·Somia S Abdel-GanySaleh A Mohamed
Dec 14, 2018·Parasites & Vectors·Yapan ZhouQijun Chen
Dec 4, 2003·Gene·Cory J Evans, Renato J Aguilera
Sep 29, 2004·Toxicon : Official Journal of the International Society on Toxinology·Kazuo ShiomiHiroshi Nagai
Sep 23, 2006·Biochimie·Marie-France Counis, Alicia Torriglia

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