Revisiting a protocol for extraction of mycobacterial lipids

International Journal of Mycobacteriology
Pooja SinghMridula Bose

Abstract

Determination of lipid content of any biological sample is essential for various kinds of studies related to pathogenicity and drug development. Thus, reliable methods for the quantitative extraction of lipids are of critical importance. The mycobacterial cell wall is largely composed of lipids. Commonly used methods to extract lipids, such as the Bligh and Dyer method or the Folch method, yield a low amount of lipids when applied to mycobacterial cells. This study presents an efficient modification of Chandramauli's method, a less known method developed at this institute earlier that is able to yield a considerably higher concentration of mycobacterial lipids.

References

Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Nov 1, 2005·Tuberculosis·Ramandeep SinghAnil K Tyagi
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Amit K Pandey, Christopher M Sassetti
Jun 3, 2009·Transboundary and Emerging Diseases·L Guenin-MacéC Demangel
Jun 23, 2009·Journal of Bacteriology·Jennifer C ChangDavid R Sherman

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