Jan 14, 2020

Revisiting the Role of Master Regulators in Tomato Ripening

Trends in Plant Science
Rufang WangRuud A de Maagd

Abstract

The study of transcriptional regulation of tomato ripening has been led by spontaneous mutations in transcription factor (TF) genes that completely inhibit normal ripening, suggesting that they are 'master regulators'. Studies using CRISPR/Cas9 mutagenesis to produce knockouts of the underlying genes indicate a different picture, suggesting that the regulation is more robust than previously thought. This requires us to revisit our model of the regulation of ripening and replace it with one involving a network of partially redundant components. At the same time, the fast rise of CRISPR/Cas mutagenesis, resulting in unexpectedly weak phenotypes, compared with knockdown technology, suggests that compensatory mechanisms may obscure protein functions. This emphasises the need for assessment of these mechanisms in plants and for the careful design of mutagenesis experiments.

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Mentioned in this Paper

Fruit Ripening
Genes
Research Study
Mutagenesis Process
Lycopersicon esculentum
Protein Function
Gene Knockdown Techniques
Transcriptional Regulation
Regulation of Biological Process
Transcription Factor

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