Revisiting the STEC Testing Approach: Using espK and espV to Make Enterohemorrhagic Escherichia coli (EHEC) Detection More Reliable in Beef

Frontiers in Microbiology
Sabine DelannoyPatrick Fach

Abstract

Current methods for screening Enterohemorrhagic Escherichia coli (EHEC) O157 and non-O157 in beef enrichments typically rely on the molecular detection of stx, eae, and serogroup-specific wzx or wzy gene fragments. As these genetic markers can also be found in some non-EHEC strains, a number of "false positive" results are obtained. Here, we explore the suitability of five novel molecular markers, espK, espV, ureD, Z2098, and CRISPRO26:H11 as candidates for a more accurate screening of EHEC strains of greater clinical significance in industrialized countries. Of the 1739 beef enrichments tested, 180 were positive for both stx and eae genes. Ninety (50%) of these tested negative for espK, espV, ureD, and Z2098, but 12 out of these negative samples were positive for the CRISPRO26:H11 gene marker specific for a newly emerging virulent EHEC O26:H11 French clone. We show that screening for stx, eae, espK, and espV, in association with the CRISPRO26:H11 marker is a better approach to narrow down the EHEC screening step in beef enrichments. The number of potentially positive samples was reduced by 48.88% by means of this alternative strategy compared to the European and American reference methods, thus substantially improving the disc...Continue Reading

References

Jul 5, 2003·Journal of Clinical Microbiology·Eva Møller Nielsen, Marianne Thorup Andersen
Apr 20, 2005·Journal of Applied Microbiology·S PerelleP Fach
Sep 20, 2005·The Journal of Infectious Diseases·John T BrooksNancy A Strockbine
Nov 17, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kristine E JohnsonCynthia L Sears
Jun 24, 2011·The New England Journal of Medicine·Christina FrankUNKNOWN HUS Investigation Team
Aug 13, 2011·Frontiers in Microbiology·Brian K CoombesChelsey D Goodman
Dec 26, 2012·Frontiers in Cellular and Infection Microbiology·Pina M Fratamico, Lori K Bagi
Feb 5, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Martina BielaszewskaHelge Karch
Jun 13, 2013·Foodborne Pathogens and Disease·Fei WangBeilei Ge
Jun 20, 2013·International Journal of Medical Microbiology : IJMM·Angelika MikoLothar Beutin
Apr 23, 2014·PloS One·Rita PragerAntje Flieger
Aug 15, 2014·Frontiers in Cellular and Infection Microbiology·Lorraine MicheletSara Moutailler
Apr 4, 2015·Journal of Food Protection·Kristin W LivezeyMichael M Becker
Jun 25, 2015·Microbiology Spectrum·Lothar Beutin, Patrick Fach

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Datasets Mentioned

BETA
CB14699

Methods Mentioned

BETA
PCR

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