Revisiting thin-layer chromatography as a lipophilicity determination tool--a comparative study on several techniques with a model solute set

Journal of Pharmaceutical and Biomedical Analysis
Łukasz KomstaMałgorzata Radoń

Abstract

The lipophilicity of a compound is a fundamental property related to pharmaceutical and biomedical activity. As many approaches are mixed together in every-day published studies, the subject needs some standardization. The paper presents a comparative study on several approaches of TLC lipophilicity determination: a single TLC run, extrapolation of a retention, principal component analysis of a retention matrix, PARAFAC on a three-way array and a PLS regression. All techniques were applied to 35 model solutes with simple molecules, using nine concentrations of six modifiers: acetonitrile, acetone, dioxane, propan-2-ol, methanol and tetrahydrofurane. The elaborated comparative analysis formed several general recommendations. Methanol and dioxane were the best modifiers, while acetonitrile gave the worst and inacceptable correlation of retention with lipophilicity. Surprisingly, good correlations were obtained for the single TLC runs and this method is underestimated in the literature. The advanced chemometric processing proposed recently, such as PCA, PARAFAC and PLS did not show a visible advantage comparing to classical methods. A need to use a robust regression and robust correlation measures, due to presence of significant o...Continue Reading

References

Feb 7, 2003·Current Medicinal Chemistry·Antoni NasalRoman Kaliszan
Jun 17, 2008·Journal of Pharmaceutical Sciences·Costas Giaginis, Anna Tsantili-Kakoulidou

❮ Previous
Next ❯

Citations

May 18, 2012·Chromatographia·Małgorzata JanickaAnna Pachuta-Stec
Dec 12, 2012·Journal of Molecular Graphics & Modelling·Veronika OpletalováKarel Palát
May 9, 2016·Journal of Pharmaceutical and Biomedical Analysis·Filip AndrićKároly Héberger
Dec 3, 2014·Journal of Pharmaceutical and Biomedical Analysis·Liyuan XuNing Li
Jul 30, 2014·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Małgorzata DołowyAlina Pyka
Jul 18, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Jovana TrifunovićMomir Mikov
Apr 12, 2014·Journal of Separation Science·Małgorzata Janicka, Anna Pachuta-Stec
Dec 9, 2017·Chemical Biology & Drug Design·Łukasz PopiołekMonika Wujec
Oct 28, 2019·International Journal of Molecular Sciences·Krzesimir CiuraJarosław Sączewski
Nov 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Krzesimir CiuraJarosław Sączewski
Aug 3, 2020·Journal of Chromatography. a·Colin F Poole, Sanka N Atapattu
Mar 7, 2021·Pharmaceuticals·Anna W SobańskaElżbieta Brzezińska
Sep 22, 2017·Journal of Chromatography. a·Krzesimir CiuraMichał J Markuszewski
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mantas VaškevičiusLiudas Šlepikas
Jul 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Łukasz PopiołekMonika Wujec
Aug 6, 2014·Journal of Agricultural and Food Chemistry·Nataša MiloševićDušan Antonović
Aug 20, 2021·Applied Microbiology and Biotechnology·Anna BiernasiukAnna Malm

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.