Revolutionizing immunology with single-cell RNA sequencing

Cellular & Molecular Immunology
Haide ChenGuoji Guo

Abstract

The immune system is composed of a complex hierarchy of cell types that protect the organism against disease and maintain homeostasis. Identifying heterogeneity of immune cells is the key to understanding the immune system. Advanced single-cell RNA sequencing (scRNA-seq) technologies are revolutionizing our ability to study immunology. By measuring transcriptomes at the single-cell level, scRNA-seq enables identification of cellular heterogeneity in far greater detail than conventional methods. In this review, we introduce the existing scRNA-seq technologies and present their strengths and weaknesses. We also discuss potential applications and future innovations of scRNA-seq in immunology.

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Methods Mentioned

BETA
flow cytometry
scRNA-seq
in vitro transcription
PCR
single-cell sequencing
laser
chip
chips
Drop-seq
inDrop

Software Mentioned

STRT
10x Genomics
Fluidigm C1
Drop
STARTRAC )
CyTOF
inDrop

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