Rewiring the Central Metabolic Pathway for High-Yield l-Serine Production in Corynebacterium glutamicum by Using Glucose

Biotechnology Journal
Xiaomei ZhangZhenghong Xu

Abstract

l-Serine is widely used in the pharmaceutical, food, and cosmetic industries. The direct fermentative production of l-serine from glucose in Corynebacterium glutamicum (C. glutamicum) has been achieved but the yield is generally low. In this study, the central metabolic pathway is rewired to direct higher accumulation of glycerate-3-phosphate (3-PG), the precursor for l-serine biosynthesis, thereby freeing up more carbon to enhance l-serine production and yield. To this end, the native phosphotransferase system (PTS) is inactivated by deleting the gene ptsH. Meanwhile, the PTS-independent glucose uptake pathway is activated via overexpression of iolT1, ppgk, and deletion of the transcriptional regulator gene iolR. Furthermore, metabolic changes between PTS and non-PTS strains are elucidated using gas chromatography-tandem mass spectrometry. Based on the metabonomic and enzyme activities analysis, the glycolysis pathway upstream of 3-PG is further enhanced by co-overexpressing pgi, pfkA, and gapA. The resulting strain with all of the modifications mentioned above leads to the l-serine yield of 0.30 g g-1 glucose (42.8% higher than that of the original strain), which is the highest yield in C. glutamicum by using glucose as the s...Continue Reading

References

Oct 31, 2002·Applied and Environmental Microbiology·Mattheos A G KoffasGregory Stephanopoulos
May 17, 2003·Metabolic Engineering·Mattheos A G KoffasGregory Stephanopoulos
Nov 5, 2005·Applied and Environmental Microbiology·Petra Peters-WendischLothar Eggeling
Dec 5, 2006·Applied and Environmental Microbiology·Michael StolzLothar Eggeling
Mar 15, 2008·Microbial Cell Factories·Judith BeckerChristoph Wittmann
Jan 7, 2009·Journal of Biotechnology·Jens-Eric SchweitzerLothar Eggeling
Apr 10, 2010·Applied Microbiology and Biotechnology·Steffen N LindnerVolker F Wendisch
Oct 20, 2011·Bioengineered Bugs·Steffen N LindnerVolker F Wendisch
May 9, 2012·Science China. Life Sciences·Shujuan LaiTingyi Wen
Aug 17, 2012·Applied Microbiology and Biotechnology·Jinlei TangXueli Zhang
Oct 20, 2012·Sub-cellular Biochemistry·Takashi HirasawaHiroshi Shimizu
May 27, 2014·Applied Biochemistry and Biotechnology·Xiaomei ZhangZhenghong Xu
Sep 10, 2014·Biotechnology and Applied Biochemistry·Tianyu QinXiaoyuan Wang
Dec 2, 2014·Applied Microbiology and Biotechnology·Qinjian ZhuZhenghong Xu
Jan 1, 2015·Applied Microbiology and Biotechnology·Masato IkedaSeiki Takeno
Feb 11, 2015·Journal of Industrial Microbiology & Biotechnology·Chuanzhi ZhangJian Chen
Apr 28, 2016·World Journal of Microbiology & Biotechnology·Volker F WendischElvira Sgobba
May 7, 2016·Current Opinion in Biotechnology·Takashi Hirasawa, Hiroshi Shimizu
Jun 28, 2016·Applied Microbiology and Biotechnology·Fernando Pérez-GarcíaVolker F Wendisch
Dec 3, 2016·Metabolic Engineering·Hemanshu MundhadaAlex T Nielsen
Feb 9, 2017·Biotechnology Journal·Fernando Pérez-GarcíaVolker F Wendisch
Oct 28, 2017·Applied Microbiology and Biotechnology·Kamila GoderskaTeresa Alarcon
Mar 4, 2018·Trends in Biotechnology·Xiaomei ZhangZhenghong Xu

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Citations

Jun 18, 2019·Biotechnology Journal·Sung Ok HanYong-Su Jin
Apr 4, 2019·Metabolic Engineering·Volker F Wendisch

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