RF-Cloning.org: an online tool for the design of restriction-free cloning projects.

Nucleic Acids Research
Stephen R Bond, Christian C Naus

Abstract

Restriction-free cloning (RF-cloning) is a PCR-based technology that expands on the QuikChange™ mutagenesis process originally popularized by Stratagene in the mid-1990s, and allows the insertion of essentially any sequence into any plasmid at any location. While RF-cloning is a powerful tool for the design of custom plasmids when restriction sites are not conveniently situated, manually designing the requisite primers can be tedious and error prone. We present here a web-service that automates the primer design process, along with a user interface that includes a number of useful tools for managing both the input sequences and the resulting outputs. RF-Cloning is free and open to all users, and can be accessed at http://www.rf-cloning.org.

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Citations

Aug 28, 2012·The Journal of Membrane Biology·Stephen R BondChristian C Naus
Mar 13, 2014·Microbial Cell Factories·Bjarte Aarmo LundGro Elin Kjæreng Bjerga
May 11, 2013·Cell Communication & Adhesion·Leigh E Wicki-StordeurLeigh Anne Swayne
Feb 9, 2016·Journal of Biotechnology·Gro Elin Kjæreng BjergaHans Torstein Kleivdal
Aug 27, 2014·Journal of Molecular Biology·Johannes G M RackRein Aasland
Jul 28, 2015·Scientific Reports·Michael H H LendersLutz Schmitt
Jul 24, 2012·Journal of Neurochemistry·Kristen BrowneTimothy P O'Connor
Apr 12, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sung In LimInchan Kwon
May 5, 2016·Nature·Meital Oren-SuissaOliver Hobert
Mar 13, 2013·BioTechniques·Ichiro Matsumura
Jun 14, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Stephen R BondJoy M Richman
Dec 23, 2016·Scientific Reports·Sung In LimInchan Kwon
Jun 15, 2017·Journal of Agricultural and Food Chemistry·Caleb R SchlachterMaksymilian Chruszcz
Apr 9, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sung In LimInchan Kwon
Dec 10, 2017·Nature Communications·Leonor MorgadoSebastian Hiller
Jun 6, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Christian KubitzaAxel J Scheidig
Apr 4, 2019·Protein Science : a Publication of the Protein Society·Ethan D McSpaddenJohn Kuriyan
Jun 4, 2019·The FEBS Journal·Jiří ZahradníkBohdan Schneider
Apr 10, 2014·PloS One·Tao WangGuanghua Xiao
Aug 23, 2019·PloS One·Metin BalabanSiavash Mirarab
Aug 5, 2020·Microbiology·Danae Morales AngelesDirk-Jan Scheffers
Aug 20, 2015·BMC Biotechnology·Gro Elin Kjæreng Bjerga, Adele Kim Williamson
Jun 2, 2018·The Journal of General Physiology·Toshiki Yamada, Kevin Strange
Dec 1, 2018·Nature Communications·Tobias WunderOliver Mueller-Cajar
Jul 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chee-Seng HeeStephan Grzesiek

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Methods Mentioned

BETA
PCR
pull-down
Destination

Software Mentioned

Apache2
Gateway®
Safari
Firefox
RF
CorelDRAW
MySQL
Adobe Illustrator
Chrome
Perl

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