PMID: 8606157Apr 1, 1996Paper

RfaH enhances elongation of Escherichia coli hlyCABD mRNA

Journal of Bacteriology
J A Leeds, R A Welch

Abstract

Escherichia coli hlyCABD operons encode the polypeptide component (Hly A) of an extracellular cytolytic toxin, as well as proteins required for its acylation (HlyC) and sec-independent secretion (HlyBD). Previous reports suggested that the E. coli protein RfaH is required for wild-type hemolysin expression, either by positively activating hly transcript initiation (M. J. A. Bailey, V. Koronakis, T. Schmoll, and C. Hughes, Mol. Microbiol. 6:1003-1012, 1992) or by promoting proper insertion of hemolysin export machinery in the E. coli outer membrane (C. Wandersman and S. Letoffe, Mol. Microbiol. 7:141-150, 1993). RfaH is also required for wild-type levels of mRNA transcribed from promoter-distal genes in the rfaQ-K, traY-Z, and rplK-rpoC gene clusters, suggesting that RfaH is a transcriptional antiterminator. We tested these models by analyzing the effects of rfaH mutations on hlyCABD mRNA synthesis and decay, HlyA protein levels, and hemolytic activity. The model system included a uropathogenic strain of E. coli harboring hlyCABD on the chromosome and E. coli K-12 transformed with the hlyCABD operon on a recombinant plasmid. Our results suggest that RfaH enhances hlyCABD transcript elongation, consistent with the model of RfaH i...Continue Reading

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Citations

May 3, 2018·Nucleic Acids Research·Beth A BoudreauRobert Landick
Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A CurtisM Rangarajan
Feb 6, 2010·Molecular Microbiology·Georgiy A BelogurovIrina Artsimovitch
Oct 20, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F M Goñi, H Ostolaza
May 7, 2010·Medical Microbiology and Immunology·Ulrich DobrindtJ Hacker
Oct 29, 2013·Journal of Bacteriology·Alexandra MooresIan C Blomfield
Oct 27, 2004·Molecular Genetics and Genomics : MGG·D Vicari, I Artsimovitch
Aug 31, 2013·Nucleic Acids Research·Sushil Kumar TomarIrina Artsimovitch
Aug 8, 2007·FEMS Microbiology Reviews·Marc LemonnierEmmanuel Lemichez
Jul 16, 2002·Infection and Immunity·Gábor NagyLevente Emödy
Jun 24, 2004·Infection and Immunity·Gábor NagyLevente Emödy
Feb 3, 2006·Journal of Bacteriology·Christophe BeloinUlrich Dobrindt
Nov 12, 2020·Structure·Allen K Kim, Lauren L Porter
May 25, 1999·Infection and Immunity·J Li, K D Clinkenbeard
Aug 25, 2015·Biochimica Et Biophysica Acta·Laura C Ristow, Rodney A Welch
Jun 1, 1997·Infection and Immunity·M E Bauer, R A Welch
Nov 1, 2005·EcoSal Plus·Rodney A Welch
Jan 1, 2006·EcoSal Plus·Jay L Mellies, Alex M S Barron
Jun 22, 2017·EcoSal Plus·James T Riordan, Avishek Mitra
Nov 1, 1996·Infection and Immunity·M E Bauer, R A Welch
May 20, 2008·International Journal of Medical Microbiology : IJMM·Gábor NagyTibor Pál

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