Rfc4 interacts with Rpa1 and is required for both DNA replication and DNA damage checkpoints in Saccharomyces cerevisiae

Molecular and Cellular Biology
H S Kim, Steven Brill

Abstract

The large subunit of replication protein A (Rpa1) consists of three single-stranded DNA binding domains and an N-terminal domain (Rpa1N) of unknown function. To determine the essential role of this domain we searched for mutations that require wild-type Rpa1N for viability in yeast. A mutation in RFC4, encoding a small subunit of replication factor C (RFC), was found to display allele-specific interactions with mutations in the gene encoding Rpa1 (RFA1). Mutations that map to Rpa1N and confer sensitivity to the DNA synthesis inhibitor hydroxyurea, such as rfa1-t11, are lethal in combination with rfc4-2. The rfc4-2 mutant itself is sensitive to hydroxyurea, and like rfc2 and rfc5 strains, it exhibits defects in the DNA replication block and intra-S checkpoints. RFC4 and the DNA damage checkpoint gene RAD24 were found to be epistatic with respect to DNA damage sensitivity. We show that the rfc4-2 mutant is defective in the G(1)/S DNA damage checkpoint response and that both the rfc4-2 and rfa1-t11 strains are defective in the G(2)/M DNA damage checkpoint. Thus, in addition to its essential role as part of the clamp loader in DNA replication, Rfc4 plays a role as a sensor in multiple DNA checkpoint pathways. Our results suggest th...Continue Reading

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Tsurimoto, B Stillman
Sep 1, 1989·Molecular and Cellular Biology·T TsurimotoB Stillman
Feb 24, 1989·Cell·B J Thomas, R Rothstein
Jan 1, 1989·Journal of Cell Science. Supplement·T Weinert, L Hartwell
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M S Wold, T Kelly
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R WobbeJ Hurwitz
Sep 1, 1995·Molecular and Cellular Biology·G CullmannB Stillman
Dec 1, 1994·Molecular and Cellular Biology·M P LongheseG Lucchini
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Li, P M Burgers
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·H ArakiA Sugino
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·K SugimotoK Matsumoto
Sep 1, 1996·Genes & Development·D PhilipovaS J Brill
Jan 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N YaoM O'Donnell
Apr 1, 1997·Current Opinion in Genetics & Development·M F Hoekstra
Mar 28, 1998·The Journal of Biological Chemistry·E BochkarevaA Bochkarev

❮ Previous
Next ❯

Citations

Dec 4, 2003·DNA Repair·Hee-Sook Kim, Steven J Brill
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jun-Hyuk ChoiAziz Sancar
Aug 2, 2001·The Journal of Biological Chemistry·S A Bastin-Shanower, S J Brill
Sep 6, 2012·The Journal of Biological Chemistry·Laura A Lindsey-BoltzAziz Sancar
Feb 5, 2008·The Journal of Biological Chemistry·Junya TomidaTakeshi Todo
May 13, 2008·The Journal of Biological Chemistry·Stuart J HaringMarc S Wold
Jun 3, 2008·The Journal of Biological Chemistry·Sara K Binz, Marc S Wold
Aug 29, 2006·Nucleic Acids Research·Ellen FanningAndrew R Nager
Dec 26, 2012·Nucleic Acids Research·Jason G GlanzerGregory G Oakley
Dec 28, 2002·Genes & Development·Kenji ShimadaSusan M Gasser
Jan 2, 2003·Molecular and Cellular Biology·Lisa A Anderson, Neil D Perkins
Jun 21, 2005·Molecular and Cellular Biology·Göran O Bylund, Peter M J Burgers
Jul 1, 2010·Molecular Cancer Research : MCR·Cathy S HassMarc S Wold
Nov 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lee ZouStephen J Elledge
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kyungjae Myung, Richard D Kolodner
Nov 11, 2008·Scandinavian Journal of Gastroenterology·Cun-Shuan XuLin Tian
May 19, 2009·Journal of Molecular Biology·Tomohiko Sugiyama, Noriko Kantake
May 22, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Masaaki AraiMikio Yamamoto
Dec 6, 2012·The EMBO Journal·Karen Kuntz, Matthew J O'Connell
Jul 29, 2004·DNA Repair·Yinhuai Chen, Yolanda Sanchez
Jul 18, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Huimin ShenNan Jiang
Nov 14, 2002·Annual Review of Genetics·Kara A NybergTed A Weinert
Dec 19, 2001·Current Biology : CB·S AhmedA Gartner
Apr 15, 2008·Molecular Cell·Jacqueline H BarlowRodney Rothstein
Jun 15, 2007·American Journal of Human Genetics·Mark O'DriscollPenny A Jeggo
May 29, 2007·DNA Repair·Renee D Paulsen, Karlene A Cimprich
Feb 22, 2017·Genes·Divya Ramalingam Iyer, Nicholas Rhind
Feb 7, 2018·The Plant Journal : for Cell and Molecular Biology·Jie QianJie Zhao
Sep 19, 2001·Yeast

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.