Abstract
We have previously shown that RGC-32 is involved in cell cycle regulation in vitro. To define the in vivo role of RGC-32, we generated RGC-32 knockout mice. These mice developed normally and did not spontaneously develop overt tumors. To assess the effect of RGC-32 deficiency on cell cycle activation in T cells, we determined the proliferative rates of CD4(+) and CD8(+) T cells from the spleens of RGC-32(-/-) mice, as compared to wild-type (WT, RGC-32(+/+)) control mice. After stimulation with anti-CD3/anti-CD28, CD4(+) T cells from RGC-32(-/-) mice displayed a significant increase in [(3)H]-thymidine incorporation when compared to WT mice. In addition, both CD4(+) and CD8(+) T cells from RGC-32(-/-) mice displayed a significant increase in the proportion of proliferating Ki67(+) cells, indicating that in T cells, RGC-32 has an inhibitory effect on cell cycle activation induced by T-cell receptor/CD28 engagement. Furthermore, Akt and FOXO1 phosphorylation induced in stimulated CD4(+) T-cells from RGC-32(-/-) mice were significantly higher, indicating that RGC-32 inhibits cell cycle activation by suppressing FOXO1 activation. We also found that IL-2 mRNA and protein expression were significantly increased in RGC-32(-/-) CD4(+) T...Continue Reading
References
Oct 1, 1993·European Journal of Immunology·S G WardD M Sansom
Oct 3, 1998·The Journal of Biological Chemistry·T C BadeaH Rus
Apr 2, 1999·Cell·A BrunetM E Greenberg
Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Nov 1, 2001·The Journal of Biological Chemistry·Tudor BadeaHorea Rus
Apr 12, 2003·Cellular Signalling·Michelle M FungKathleen L McGuire
May 30, 2003·The Journal of Biological Chemistry·Michael PotenteIngrid Fleming
Jul 29, 2003·European Journal of Immunology·Jeffrey C RathmellCraig B Thompson
Feb 17, 2005·Experimental and Molecular Pathology·Matthew FosbrinkHorea Rus
Jun 4, 2005·Clinical and Experimental Immunology·T TanakaT Hara
May 4, 2006·The Journal of Biological Chemistry·Matthew FosbrinkHorea Rus
Sep 5, 2006·Cancer Metastasis Reviews·Sonia B Jakowlew
Jul 31, 2007·Experimental and Molecular Pathology·Cornelia CudriciHorea Rus
Mar 1, 2008·Endocrinology·Eun-Sil ParkMisung Jo
Apr 1, 2008·Archivum Immunologiae Et Therapiae Experimentalis·Sonia I VlaicuHorea Rus
Apr 9, 2008·Current Opinion in Cell Biology·Dervis A M Salih, Anne Brunet
Jun 19, 2008·Oncogene·S WottonJ C Neil
Aug 12, 2008·Journal of Neuroimmunology·Cornelia CudriciHorea Rus
Jan 24, 2009·Experimental and Molecular Pathology·Matthew FosbrinkHorea Rus
Aug 5, 2009·Circulation·Xiaojin AnJian Li
Nov 4, 2009·Experimental and Molecular Pathology·Sonia I VlaicuHorea Rus
Jun 29, 2011·Biochimica Et Biophysica Acta·Guri TzivionGopalakrishnan Ramakrishnan
Sep 25, 2012·Experimental and Molecular Pathology·Cosmin A TeglaHorea Rus
May 23, 2013·Cardiovascular Research·Xiao-Bing CuiShi-You Chen
Citations
Jan 13, 2016·Immunologic Research·Sonia I VlaicuHorea Rus
Sep 27, 2015·Experimental and Molecular Pathology·Adam M KruszewskiHorea Rus
Sep 14, 2016·Experimental and Molecular Pathology·Sonia I VlaicuHorea Rus
Dec 13, 2016·Cell Transplantation·Scott E Counts, Elliott J Mufson
Feb 1, 2018·Nucleic Acids Research·Michèle BrocardMichelle J West
Mar 31, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Violeta RusHorea G Rus
Aug 25, 2018·PloS One·Hyeonsoo JungSang-Beom Seo
May 26, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Xiao-Bing CuiShi-You Chen
Apr 18, 2019·Bioinformatics·Justin D Finkle, Neda Bagheri
Feb 23, 2020·Nature Communications·Todd BradleyBarton F Haynes
Oct 4, 2018·Frontiers in Cardiovascular Medicine·Xiao-Bing Cui, Shi-You Chen
Jun 30, 2019·Immunologic Research·Sonia I VlaicuHorea Rus
Oct 12, 2019·Cell Death & Disease·Peng ZhaoYan Liu
Mar 20, 2018·Scientific Reports·Anny Waloski RobertMarco Augusto Stimamiglio
Jul 15, 2018·Immunologic Research·Alexandru TatomirHorea Rus
Mar 9, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sarah L BuchanAymen Al-Shamkhani
May 28, 2020·European Journal of Immunology·Natania S FieldPaula M Oliver
Feb 12, 2021·Frontiers in Immunology·Alexandru TatomirHorea Rus
May 29, 2021·BMC Cancer·Lee-Chin ChanSuet-Lin Chia
Jul 30, 2021·EMBO Reports·Zhenming GuoGuang He
Aug 17, 2021·Frontiers in Immunology·Alexandru TatomirHorea Rus
Aug 20, 2021·American Journal of Reproductive Immunology : AJRI·Jessica VazquezAleksandar K Stanic