RGC-32 is a novel regulator of the T-lymphocyte cell cycle

Experimental and Molecular Pathology
Cosmin A TeglaHorea Rus

Abstract

We have previously shown that RGC-32 is involved in cell cycle regulation in vitro. To define the in vivo role of RGC-32, we generated RGC-32 knockout mice. These mice developed normally and did not spontaneously develop overt tumors. To assess the effect of RGC-32 deficiency on cell cycle activation in T cells, we determined the proliferative rates of CD4(+) and CD8(+) T cells from the spleens of RGC-32(-/-) mice, as compared to wild-type (WT, RGC-32(+/+)) control mice. After stimulation with anti-CD3/anti-CD28, CD4(+) T cells from RGC-32(-/-) mice displayed a significant increase in [(3)H]-thymidine incorporation when compared to WT mice. In addition, both CD4(+) and CD8(+) T cells from RGC-32(-/-) mice displayed a significant increase in the proportion of proliferating Ki67(+) cells, indicating that in T cells, RGC-32 has an inhibitory effect on cell cycle activation induced by T-cell receptor/CD28 engagement. Furthermore, Akt and FOXO1 phosphorylation induced in stimulated CD4(+) T-cells from RGC-32(-/-) mice were significantly higher, indicating that RGC-32 inhibits cell cycle activation by suppressing FOXO1 activation. We also found that IL-2 mRNA and protein expression were significantly increased in RGC-32(-/-) CD4(+) T...Continue Reading

References

Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Nov 1, 2001·The Journal of Biological Chemistry·Tudor BadeaHorea Rus
Apr 12, 2003·Cellular Signalling·Michelle M FungKathleen L McGuire
Feb 17, 2005·Experimental and Molecular Pathology·Matthew FosbrinkHorea Rus
Jun 4, 2005·Clinical and Experimental Immunology·T TanakaT Hara
Sep 5, 2006·Cancer Metastasis Reviews·Sonia B Jakowlew
Jul 31, 2007·Experimental and Molecular Pathology·Cornelia CudriciHorea Rus
Apr 1, 2008·Archivum Immunologiae Et Therapiae Experimentalis·Sonia I VlaicuHorea Rus
Apr 9, 2008·Current Opinion in Cell Biology·Dervis A M Salih, Anne Brunet
Jan 24, 2009·Experimental and Molecular Pathology·Matthew FosbrinkHorea Rus
Nov 4, 2009·Experimental and Molecular Pathology·Sonia I VlaicuHorea Rus
Jun 29, 2011·Biochimica Et Biophysica Acta·Guri TzivionGopalakrishnan Ramakrishnan
Sep 25, 2012·Experimental and Molecular Pathology·Cosmin A TeglaHorea Rus
May 23, 2013·Cardiovascular Research·Xiao-Bing CuiShi-You Chen

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Citations

Jan 13, 2016·Immunologic Research·Sonia I VlaicuHorea Rus
Sep 27, 2015·Experimental and Molecular Pathology·Adam M KruszewskiHorea Rus
Dec 13, 2016·Cell Transplantation·Scott E Counts, Elliott J Mufson
Mar 31, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Violeta RusHorea G Rus
Feb 23, 2020·Nature Communications·Todd BradleyBarton F Haynes
Oct 4, 2018·Frontiers in Cardiovascular Medicine·Xiao-Bing Cui, Shi-You Chen
Jun 30, 2019·Immunologic Research·Sonia I VlaicuHorea Rus
Mar 20, 2018·Scientific Reports·Anny Waloski RobertMarco Augusto Stimamiglio
Mar 9, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sarah L BuchanAymen Al-Shamkhani
May 28, 2020·European Journal of Immunology·Natania S FieldPaula M Oliver
Feb 12, 2021·Frontiers in Immunology·Alexandru TatomirHorea Rus
Aug 20, 2021·American Journal of Reproductive Immunology : AJRI·Jessica VazquezAleksandar K Stanic

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