RGC Neuroprotection Following Optic Nerve Trauma Mediated By Intranasal Delivery of Amnion Cell Secretome

Investigative Ophthalmology & Visual Science
Gabriela A GrinblatKenneth S Shindler

Abstract

Intranasally delivered ST266, the biological, proteinaceous secretome of amnion-derived multipotent progenitor cells, reduces retinal ganglion cell (RGC) loss, optic nerve inflammation, and demyelination in experimental optic neuritis. This unique therapy and novel administration route delivers numerous cytokines and growth factors to the eye and optic nerve, suggesting a potential to also treat other optic neuropathies. Thus, ST266-mediated neuroprotection was examined following traumatic optic nerve injury. Optic nerve crush injury was surgically induced in C57BL/6J mice. Mice were treated daily with intranasal PBS or ST266. RGC function was assessed by optokinetic responses (OKRs), RGCs were counted, and optic nerve sections were stained with luxol fast blue and anti-neurofilament antibodies to assess myelin and RGC axon damage. Intranasal ST266 administered daily for 5 days, beginning at the time that a 1-second optic nerve crush was performed, significantly attenuated OKR decreases. Furthermore, ST266 treatment reduced damage to RGC axons and myelin within optic nerves, and blocked RGC loss. Following a 4-second optic nerve crush, intranasal ST266 increased RGC survival and showed a trend toward reduced RGC axon and myelin...Continue Reading

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Citations

Mar 5, 2019·Journal of Neuro-ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society·Reas S KhanKenneth S Shindler
Nov 10, 2020·Veterinary Ophthalmology·Paul E Miller, Joshua Seth Eaton
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Rebecca M HarmanGerlinde R Van de Walle

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Methods Mentioned

BETA
dissecting

Software Mentioned

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GraphPad
Pro Plus
ImageJ
OptoMotry
GraphPad Prism

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