RGNNV induces mitochondria-mediated cell death via newly synthesized protein dependent pathway in fish cells

Fish & Shellfish Immunology
Horng-Cherng WuJiann-Ruey Hong

Abstract

The RNA nervous necrosis virus induces necrotic cell death in fish; however, the molecular mechanism remains unknown. In this study, we demonstrated that beta-nodavirus-induced mitochondria-mediated dependent cell death is through newly synthesized protein dependent pathway in replication cycle. We determined that newly synthesized protein dependent pathway is required for red-spotted grouper nervous necrosis virus (RGNNV)-induced cell death. UV irradiation of the virus effectively blocked viral replication and cell death. Next, RGNNV RNA-dependent RNA polymerase (RdRp or protein A) was cloned and its involvement in RNA genome replication and viral protein synthesis was analyzed. Protein A was initially expressed 48 h post-infection and localized to the cytoplasm. Knockdown of protein A expression completely blocked viral genomic replication and expression of viral protein expression RNA1 small hairpin RNA (shRNA) producing cell lines, which coincided with inhibition of phosphatidylserine exposure, mitochondria-mediated death signaling, and increased cell viability 72 h post-infection. Furthermore, RGNNV-induced mitochondria-mediated caspase-3-independent necrotic cell death is dependent on viral synthesized protein dependent p...Continue Reading

Citations

Dec 21, 2010·Apoptosis : an International Journal on Programmed Cell Death·Yu-Chin SuJiann-Ruey Hong
Aug 30, 2014·Critical Reviews in Microbiology·Shanshan LiXilan Yu
Feb 3, 2016·Apoptosis : an International Journal on Programmed Cell Death·Latif ReshiJiann-Ruey Hong
Jun 20, 2014·Fish & Shellfish Immunology·Mohammad Latif ReshiJiann-Ruey Hong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.