RGS2 is regulated by angiotensin II and functions as a negative feedback of aldosterone production in H295R human adrenocortical cells.

Endocrinology
Damian G RomeroCelso E Gomez-Sanchez

Abstract

Regulator of G protein signaling (RGS) proteins interact with Galpha-subunits of heterotrimeric G proteins, accelerating the rate of GTP hydrolysis and finalizing the intracellular signaling triggered by the G protein-coupled receptor-ligand interaction. Angiotensin (Ang) II interacts with its G protein-coupled receptor in zona glomerulosa adrenal cells and triggers a cascade of intracellular signals that regulates steroidogenesis and proliferation. We studied Ang II-mediated regulation of RGS2, the role of RGS2 in steroidogenesis, and the intracellular signal events involved in H295R human adrenal cells. We report that both H295R cells and human adrenal gland express RGS2 mRNA. In H295R cells, Ang II caused a rapid and transient increase in RGS2 mRNA levels quantified by real-time RT-PCR. Ang II effects were mimicked by calcium ionophore A23187 and blocked by calcium channel blocker nifedipine. Ang II effects also were blocked by calmodulin antagonists (W-7 and calmidazolium) and calcium/calmodulin-dependent kinase antagonist KN-93. RGS2 overexpression by retroviral infection in H295R cells caused a decrease in Ang II-stimulated aldosterone secretion but did not modify cortisol secretion. In reporter assays, RGS2 decreased Ang...Continue Reading

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Citations

Oct 29, 2010·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Shizuka AritomiMichihiro Yoshimura
May 20, 2011·American Journal of Physiology. Cell Physiology·Jagadambika J GunajeWilliam M Mahoney
May 1, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Jacob KachNickolai O Dulin
Sep 30, 2010·Physiological Genomics·Damian G RomeroCelso E Gomez-Sanchez
Apr 9, 2010·Journal of Hypertension·Andrea SempliciniGiulio Ceolotto
Sep 6, 2007·Drug Metabolism Reviews·Marion B SewerSrinath Jagarlapudi
Jan 12, 2007·Molecular and Cellular Endocrinology·Jean-Guy Lehoux, Andrée Lefebvre
May 12, 2009·Molecular and Cellular Endocrinology·Licy L Yanes, Damian G Romero
Sep 25, 2008·Molecular and Cellular Endocrinology·Edson F NogueiraWilliam E Rainey
Nov 17, 2007·Pharmacology & Therapeutics·Geetanjali BansalZhihui Xie
Jan 12, 2010·Biochimica Et Biophysica Acta·Henriette L Brinks, Andrea D Eckhart
Mar 1, 2007·Physiological Genomics·Damian G RomeroCelso E Gomez-Sanchez
Oct 16, 2008·American Journal of Physiology. Endocrinology and Metabolism·Tanja Brenner, Kevin M O'Shaughnessy
Oct 3, 2014·Pathology International·Yasuhiro NakamuraHironobu Sasano
Dec 25, 2009·American Journal of Physiology. Cell Physiology·Mehari EndaleMan Hee Rhee
Feb 17, 2007·Endocrinology·Damian G RomeroCelso E Gomez-Sanchez
Jan 9, 2021·Function·Dingguo ZhangDavid M Pollock
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity

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