RGS2: regulation of expression and nuclear localization

Biochemical and Biophysical Research Communications
L SongR S Jope

Abstract

RGS2, a Regulators of G-protein Signaling family member, regulates signaling activities of G-proteins, and RGS2 itself is controlled in part by regulation of its expression. This investigation extended previous studies of the regulation of RGS2 expression by examining the effects of stress, differentiation, and signaling activities on RGS2 mRNA level in human neuroblastoma SH-SY5Y cells. Cell stress induced by heat shock rapidly and transiently increased RGS2 mRNA levels, whereas differentiation to a neuronal phenotype reduced basal RGS2 mRNA levels by 50%. RGS2 mRNA levels were increased in differentiated cells by heat shock, carbachol, and activation of protein kinase C. After transient transfection of GFP-tagged RGS2, a predominant nuclear localization was observed by confocal microscopy. Thus, RGS2 expression is regulated by stress and differentiation, as well as by second messenger signaling, and transfected GFP-RGS2 is predominantly nuclear.

References

Feb 14, 1997·The Journal of Biological Chemistry·H G Dohlman, J Thorner
Jan 27, 1998·The Journal of Biological Chemistry·D M Berman, A G Gilman
Apr 6, 2000·The Journal of Biological Chemistry·N O DulinM J Dunn
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·L De VriesM G Farquhar
Jun 28, 2000·The Journal of Biological Chemistry·T K Chatterjee, R A Fisher

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Citations

May 1, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Jacob KachNickolai O Dulin
Dec 29, 2013·Archives of Oral Biology·Rui Mauricio Santos de AraujoKeiji Moriyama
May 31, 2006·Neuroscience Letters·Ling Song, Richard S Jope
Dec 25, 2009·American Journal of Physiology. Cell Physiology·Mehari EndaleMan Hee Rhee
Jun 22, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ariadne LegendreRuth B S Harris
Feb 13, 2007·Cell Biochemistry and Function·Rui M Santos de AraujoKeiji Moriyama

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