RgsA, an RpoS-dependent sRNA, negatively regulates rpoS expression in Pseudomonas aeruginosa

Microbiology
Pei LuShiyun Chen

Abstract

As a master regulator, the alternative sigma factor RpoS coordinates the transcription of genes associated with protection against environmental stresses in bacteria. In Pseudomonas aeruginosa, RpoS is also involved in quorum sensing and virulence. The cellular RpoS level is regulated at multiple levels, whereas the post-transcriptional regulation of rpoS in P. aeruginosa remains unclear. To identify and characterize small regulatory RNAs (sRNAs) regulating RpoS in P. aeruginosa, an sRNA library expressing a total of 263 sRNAs was constructed to examine their regulatory roles on rpoS expression. Our results demonstrate that rpoS expression is repressed by the RpoS-dependent sRNA RgsA at the post-transcriptional level. Unlike OxyS, an sRNA previously known to repress rpoS expression under oxidative stress in Escherichia coli, RgsA represses rpoS expression during the exponential phase. This repression requires the RNA chaperone Hfq. Furthermore, the 71-77 conserved region of RgsA is necessary for full repression of rpoS expression, and the -25 to +27 region of rpoS mRNA is sufficient for RgsA-mediated rpoS repression. Together, our results not only add RgsA to the RpoS regulatory circuits but also highlight the complexity of int...Continue Reading

References

Mar 1, 1996·Journal of Bacteriology·D L MiltonH Wolf-Watz
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·N MajdalaniS Gottesman
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·C BlumerD Haas
Jan 24, 2002·Molecular Cell·Aixia ZhangGisela Storz
Mar 27, 2002·Journal of Bacteriology·Milan KojicVittorio Venturi
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Oct 3, 2003·Microbial Pathogenesis·Elisabeth SonnleitnerUdo Bläsi
Feb 7, 2004·Molecular Microbiology·Martin SchusterE P Greenberg
Sep 24, 2004·RNA·Marc RehmsmeierRobert Giegerich
Feb 1, 2007·Nucleic Acids Research·Johannes H Urban, Jörg Vogel
Apr 15, 2008·BMC Genomics·Nicolas GonzálezDieter Haas
Sep 8, 2009·Trends in Microbiology·Ambre JousselinBrice Felden
Dec 2, 2009·Infection and Immunity·Tao Dong, Herb E Schellhorn
Sep 24, 2010·Nature Reviews. Molecular Cell Biology·Alison Schuldt
Oct 29, 2010·Cold Spring Harbor Perspectives in Biology·Susan Gottesman, Gisela Storz
Jun 7, 2011·Annual Review of Microbiology·Aurelia BattestiSusan Gottesman
Jul 16, 2011·Nature Reviews. Microbiology·Jörg Vogel, Ben F Luisi
Sep 6, 2011·Biochimica Et Biophysica Acta·Taylor B Updegrove, Roger M Wartell
Sep 20, 2011·Molecular Cell·Gisela StorzKaren M Wassarman
Apr 27, 2012·Environmental Microbiology·María Gómez-LozanoKatherine S Long
Aug 29, 2012·Molecular Microbiology·Nicholas De Lay, Susan Gottesman
Sep 5, 2013·Cold Spring Harbor Perspectives in Medicine·Isabelle CaldelariJörg Vogel
Aug 25, 2015·Advances in Genetics·E Gerhart H Wagner, Pascale Romby
Feb 10, 2016·Current Microbiology·Jesús Manuel HuertaMiguel Castañeda
May 4, 2016·Nucleic Acids Research·Hyun-Jung Lee, Susan Gottesman
Jul 7, 2016·Molecular Microbiology·Pei LuShiyun Chen
Nov 1, 2016·Nature Reviews. Microbiology·Lionello Bossi, Nara Figueroa-Bossi
Sep 24, 2016·Cell·Nadezda SedlyarovaEvgeny Nudler
Nov 11, 2017·Current Opinion in Microbiology·Kumari KavitaSusan Gottesman

❮ Previous
Next ❯

Citations

Apr 24, 2019·Journal of Bacteriology·Emily A Williams McMackinTimothy L Yahr
Nov 14, 2018·Frontiers in Microbiology·Hue Thi Bach NguyenUdo Bläsi
Nov 30, 2018·International Journal of Molecular Sciences·Tiago PitaJorge H Leitão
Jun 30, 2021·Current Microbiology·Shuyi HouXiuyu Song

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.