PMID: 22586915May 17, 2012Paper

Rhamnolipid production by Pseudomonas aeruginosa engineered with the Vitreoscilla hemoglobin gene

Prikladnaia biokhimiia i mikrobiologiia
Huseyin Kahraman, S O Erenler

Abstract

The potential of Pseudomonas aeruginosa expressing the Vitreoscilla hemoglobin gene (vgb) for rhamnolipid production was studied. P. aeruginosa (NRRL B-771) and its transposon mediated vgb transferred recombinant strain, PaJC, were used in the research. The optimization of rhamnolipid production was carried out in the different conditions of cultivation (agitation rate, the composition of culture medium and temperature) in a time-course manner. The nutrient source, especially the carbon type, had a dramatic effect on rhamnolipid production. The PaJC strain and the wild type cells of P. aeruginosa started producing biosurfactant at the stationary phase and its concentration reached maximum at 24 h (838 mg/l(-1)) and at 72 h (751 mg l(-1)) of the incubation respectively. Rhamnolipid production was optimal in batch cultures when the temperature and agitation rate were controlled at 30 degrees C and 100 rpm. It reached 8373 mg l(-1) when the PaJC cells were grown in 1.0% glucose supplemented minimal media. Genetic engineering of biosurfactant producing strains with vgb may be an effective method to increase its production.

References

Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·U A Ochsner, J Reiser
Aug 5, 1998·Current Opinion in Biotechnology·E R Sullivan
Mar 17, 1999·Applied Microbiology and Biotechnology·S Lang, D Wullbrandt
May 5, 2005·Applied and Environmental Microbiology·Nereus W GuntherDaniel K Y Solaiman
Sep 15, 2005·Applied Microbiology and Biotechnology·Gloria Soberón-ChávezEric Déziel
Oct 21, 2005·Journal of Bacteriology·Nicky C CaiazzaG A O'Toole
Dec 14, 2005·Applied Microbiology and Biotechnology·Amedea PerfumoRoger Marchant
Feb 14, 2006·The Journal of Antimicrobial Chemotherapy·Lígia RodriguesRosário Oliveira
Feb 24, 2006·Journal of Industrial Microbiology & Biotechnology·Ilhan DoganBenjamin C Stark
Mar 27, 2007·Chemistry and Physics of Lipids·Safi A MonteiroNadia Krieger
Mar 8, 2008·The Journal of General and Applied Microbiology·Wichuda KatemaiAran H-Kittikun

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Citations

Jul 6, 2014·Current Opinion in Biotechnology·Robin GeysInge Van Bogaert
Sep 27, 2016·The Journal of General and Applied Microbiology·Ruyi Sha, Qin Meng
Dec 15, 2017·Biotechnology and Bioengineering·Guansheng LiuZhifeng Liu
Aug 7, 2017·Microbial Cell Factories·Huiqing Chong, Qingxin Li

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