PMID: 40956Nov 1, 1979

Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme

Journal of Bacteriology
A Boronat, J Aguilar

Abstract

Escherichia coli are capable of growing anaerobically on L-rhamnose as a sole source of carbon and energy and without any exogenous hydrogen acceptor. When grown under such condition, synthesis of a nicotinamide adenine dinucleotide-linked L-lactaldehydepropanediol oxidoreductase is induced. The functioning of this enzyme results in the regeneration of nicotinamide adenine dinucleotide. The enzyme was purified to electrophoretic homogeneity. It has a molecular weight of 76,000, with two subunits that are indistinguishable by electrophoretic mobility. The enzyme reduces L-lactaldehyde to L-1,2-propanediol with reduced nicotinamide adenine dinucleotide as a cofactor. The Km were 0.035 mM L-lactaldehyde and 1.25 mM L-1,2-propanediol, at pH 7.0 and 9.5, respectively. The enzyme acts only on the L-isomers. Strong substrate inhibition was observed with L-1,2-propanediol (above 25 mM) in the dehydrogenase reaction. The enzyme has a pH optimum of 6.5 for the reduction of L-lactaldehyde and of 9.5 for the dehydrogenation of L-1,2-propanediol. The enzyme is, according to the parameters presented in this report, indistinguishable from the propanediol oxidoreductase induced by anaerobic growth on fucose.

References

Sep 17, 2008·The FEBS Journal·Seiya WatanabeKeisuke Makino
Mar 23, 2011·Applied Microbiology and Biotechnology·Satoko NiimiHideaki Yukawa
Jul 13, 2013·The Biochemical Journal·Joana Sá-PessoaMargarida Casal
May 9, 1998·The Journal of Biological Chemistry·Z LuE C Lin
Aug 31, 2013·The Journal of Microbiology·Changhan LeeChankyu Park
Dec 18, 1991·European Journal of Biochemistry·F X QuintillaJ Aguilar
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Related Concepts

Rhamnose, L-Isomer
Alkalescens-Dispar Group
Alcohol Oxidoreductases
Propylene Glycol
Lactaldehyde
Propylene Glycols
Substrate Specificity
Purification Aspects
Metabolic Inhibition
Natural Regeneration

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