Rhaponticum carthamoides transformed root extract inhibits human glioma cells viability, induces double strand DNA damage, H2A.X phosphorylation, and PARP1 cleavage

Cytotechnology
Ewa SkałaPrzemysław Sitarek

Abstract

Rhaponticum carthamoides transformed root extract induces double strand DNA damage by increasing the number of phosphorylated H2A.X- and cleaved PARP1-positive U87MG cells and patient-derived IV grade glioma cells. Furthermore, treatment of these cells with root extract causes down-regulation of UHRF1 and DNMT1. Transformed root extract is rich in caffeoylquinic acid derivatives, especially tricaffeoylquinic acid derivatives. Our findings demonstrate that the R. carthamoides transformed root extract may trigger apoptosis in glioma cells by induction of DNA damage, PARP cleavage and epigenetic modification.

References

Jan 1, 2003·Bioscience, Biotechnology, and Biochemistry·Makoto YoshimotoOsamu Yamakawa
Sep 6, 2005·Genes & Development·Mi Young KimW Lee Kraus
May 12, 2006·Cell Cycle·Margaret Nieborowska-SkorskaTomasz Skorski
Jan 4, 2007·Journal of Agricultural and Food Chemistry·Rie KurataMakoto Yoshimoto
Nov 3, 2007·Genes & Development·Frank B FurnariWebster K Cavenee
May 22, 2009·Phytochemistry·Ladislav Kokoska, Dagmar Janovska
Jan 11, 2011·The Biochemical Journal·Amy L TienChinweike Ukomadu
Jun 13, 2012·Cancer Cell International·Wissem BhouriLeila Chekir Ghedira
Jul 17, 2012·Journal of Agricultural and Food Chemistry·Estefanía Burgos-MorónMiguel López-Lázaro
May 9, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Benjamin DemoulinPatrick Dumont
Jul 30, 2015·International Journal of Biological Sciences·Muhammad KhanTonghui Ma
Sep 13, 2015·Apoptosis : an International Journal on Programmed Cell Death·Hala Gali-MuhtasibNadine Darwiche
Apr 14, 2016·Journal of Medicinal Food·Charlette TilokeAnil A Chuturgoon
Jul 15, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eva Brglez MojzerUrban Bren
Dec 3, 2016·Nutrition Journal·Preethi G AnantharajuSubbaRao V Madhunapantula
Jan 18, 2017·Phytotherapy Research : PTR·Akash TariqMuhammad Adnan
Feb 22, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Harsimran Sidhu, Neena Capalash
Jan 1, 2013·3 Biotech·Priya Batra, Anil K Sharma

❮ Previous
Next ❯

Citations

Oct 21, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M N AbbasH Cui

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
FACS
Assay
PCR
flow cytometry

Software Mentioned

MxPro
STATISTICA

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis