RHD genotyping in weak D phenotypes by multiple polymerase chain reactions

Transfusion
T J LeglerS Panzer

Abstract

Weak D phenotypes involve a quantitative variation of D. The genomic basis in weak D has been disputed, however. Five sequence-specific polymerase chain reactions (SSP-PCRs) on exons 2, 5, and 7 of the RHD gene were evaluated in 248 white and 98 Japanese blood donors and compared with the results obtained by amplification of intron 4 and serology. All methods and SSP-PCR testing on the 3' non-coding region of the RHD gene were applied to the genotyping of 94 DNA samples derived from individuals expressing weak D phenotypes. Concordant results were obtained with all genotyping and phenotyping methods in testing 201 D-positive and 145 D-negative donors. Four of 94 weak D samples were typed as D-negative by amplification of intron 4 and SSP-PCR on exon 5. Phenotyping with monoclonal antibodies revealed a DVI category in one of these cases and DFR phenotype in three of these cases. One weak D sample, which reacted like normal D-positive cells with all applied monoclonal antibodies, was typed falsely negative by SSP-PCR on exon 5 because of a point mutation at nucleotide 667 (T-->G) that resulted in a Phe223Val amino acid substitution. In this individual, heterozygosity was found at two other amino acid positions (Glu233Gln and Val2...Continue Reading

Citations

Nov 6, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·F Y Lau, G Cheng
Jun 6, 2000·Vox Sanguinis·T J LeglerM Köhler
Dec 3, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Awatef SassiSaloua Jemni Yacoub
Dec 25, 2013·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·M OuchariS Jemni Yacoub
Nov 16, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Marli Aparecida Luvisuto Rossett FlôresAna Maria Sell
Oct 19, 2005·Prenatal Diagnosis·Frederik Banch ClausenMorten Hanefeld Dziegiel
Dec 10, 1999·Cytometry·G Garratty, P A Arndt
Oct 21, 2017·Therapeutic Advances in Hematology·Gregory A DenommeWilly A Flegel
Dec 15, 2020·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Melek YanasikFiliz Aydin

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