Rhein inhibits ATP-triggered inflammatory responses in rheumatoid rat fibroblast-like synoviocytes

International Immunopharmacology
Fen HuJingjun Xu

Abstract

Rheumatoid arthritis (RA) is a chronic and systemic inflammatory disorder, which may lead to joint disabilities. So far the pathogenesis of RA remains largely undetermined, and there are still no potent drugs for clinical treatment. Rhein, a natural bioactive anthraquinone derivative, exhibited significant anti-inflammatory activities demonstrated by previous studies. Here we aimed to investigate the effects of rhein on ATP-induced inflammation responses in fibroblast-like synoviocytes isolated from a rat model of collagen induced arthritis (CIA). Our results showed that ATP triggered rapid cytosolic calcium concentration ([Ca2+]c) increase depending on extracellular Ca2+ entry. Given the major P2 subtypes expressed in rat synoviocytes were P2X4 and P2Y2 receptors, ATP-elicited calcium entry should be mainly resulted from activating P2X4. Interestingly, rhein could effectively block the ATP-induced [Ca2+]c increases in a dose-dependent manner. Besides, rhein also suppressed the production of intracellular reactive oxygen species (ROS) induced by ATP in synoviocytes that was resulted from P2X4-mediated Ca2+ entry. Brilliant blue G (BBG), which can block P2X4 receptor at high concentration, showed similar suppressive effects on a...Continue Reading

Citations

Jan 31, 2021·European Journal of Pharmacology·Li ChengJingkao Chen
Aug 24, 2020·Fitoterapia·Paulo Anastácio Furtado PachecoRobson Xavier Faria
Jul 2, 2021·Journal of Ethnopharmacology·Yuan WangYan-Xu Chang
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Jiahui LuoKai Sun

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