Rheological analysis and measurement of neutrophil indentation

Biophysical Journal
E LomakinaR E Waugh

Abstract

Aspects of neutrophil mechanical behavior relevant to the formation of adhesive contacts were assessed by measuring the dependence of the contact area between the cell and a spherical substrate under controlled loading. Micropipettes were used to bring neutrophils into contact with spherical beads under known forces, and the corresponding contact area was measured over time. The neutrophil was modeled as a viscous liquid drop with a constant cortical tension. Both the equilibrium state and the dynamics of the approach to equilibrium were examined. The equilibrium contact area increased monotonically with force in a manner consistent with a cell cortical tension of 16-24 pN/microm. The dynamic response matched predictions based on a model of the cell as a growing drop using published values for the effective viscosity of the cell. The contact pressure between the cell and substrate at equilibrium is predicted to depend on the curvature of the contacting substrate, but to be independent of the impingement force. The approach to equilibrium was rapid, such that the time-averaged stress for a two-second impingement was within 20% of the equilibrium value. These results have implications for the role of mechanical force in the forma...Continue Reading

References

Sep 21, 1992·Journal of Theoretical Biology·C Dong, R Skalak
Jun 1, 1992·Biophysical Journal·D Needham, R M Hochmuth
Aug 1, 1990·Journal of Biomechanical Engineering·D Needham, R M Hochmuth
Jun 22, 1988·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M DemboD Hammer
Oct 1, 1981·Biophysical Journal·G W Schmid-SchönbeinS Chien
Mar 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S L ErlandsenR D Nelson
Nov 1, 1993·Biophysical Journal·M A TsaiR E Waugh
Aug 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R E BruehlD F Bainton
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J Y ShaoR M Hochmuth
Jun 22, 1999·Annals of Biomedical Engineering·C DongH H Lipowsky
Jan 14, 2000·Journal of Biomechanical Engineering·X LeiC Dong
Mar 30, 2001·The Journal of Biological Chemistry·T E WilliamsC Zhu
Nov 27, 2002·Annals of Biomedical Engineering·C SpillmannR Waugh
Apr 30, 2003·Biophysical Journal·Marc HerantMicah Dembo
Sep 14, 2004·Biophysical Journal·C M SpillmannR E Waugh

❮ Previous
Next ❯

Citations

Feb 25, 2010·Biomechanics and Modeling in Mechanobiology·E H ZhouC T Lim
Mar 28, 2008·Nature Cell Biology·M KriegC-P Heisenberg
Aug 9, 2012·Trends in Cell Biology·Guillaume SalbreuxEwa Paluch
Jul 22, 2008·Respiratory Physiology & Neurobiology·Atsushi Shirai
May 24, 2008·Computers in Biology and Medicine·Vijay Pappu, Prosenjit Bagchi
Jul 4, 2008·Journal of Theoretical Biology·Vijay PappuProsenjit Bagchi
Aug 20, 2015·Biophysical Journal·Steven J HenryDaniel A Hammer
Jul 28, 2015·Biochimica Et Biophysica Acta·Andrew E EkpenyongJochen Guck
Dec 16, 2010·Biophysical Journal·Suman BoseRohit Karnik
May 23, 2014·Current Biology : CB·Andrew G ClarkEwa K Paluch
Nov 13, 2009·Current Biology : CB·Ewa Paluch, Carl-Philipp Heisenberg
Jan 31, 2006·Biophysical Journal·Michael J RosenbluthDaniel A Fletcher
Aug 8, 2006·Biophysical Journal·Pere Roca-CusachsDaniel Navajas
Jul 8, 2009·Biophysical Journal·Sandrine A HocdéRichard E Waugh
Sep 14, 2004·Biophysical Journal·C M SpillmannR E Waugh
Nov 5, 2016·Molecular Biology of the Cell·Lionel GuillouJulien Husson
Jan 21, 2016·Development·Kaoru SugimuraFrançois Graner
Jan 15, 2015·Annals of Biomedical Engineering·Michael T BesteRichard E Waugh
Nov 12, 2016·The EMBO Journal·Amayra Hernández-VegaEnrique Martín-Blanco
Dec 8, 2011·Physical Biology·Masashi Fujita, Shuichi Onami
Nov 9, 2016·The Analyst·Kisoo KimWon Gu Lee
Mar 7, 2020·Biophysical Journal·Luis F DelgadilloRichard E Waugh
Oct 17, 2015·Journal of Cell Science·Rudolf Winklbauer
Jun 23, 2021·Analytical Methods : Advancing Methods and Applications·Jiajing ZhuXianping Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
J Y ShaoR M Hochmuth
© 2021 Meta ULC. All rights reserved