Rheology of perfusates and fluid dynamical effects during whole organ decellularization: a perspective to individualize decellularization protocols for single organs

Biofabrication
Jörn HülsmannPayam Akhyari

Abstract

The approach of whole organ decellularization is rapidly becoming more widespread within the tissue engineering community. Today it is well known that the effects of decellularization protocols may vary with the particular type of treated tissue. However, there are no methods known to individualize decellularization protocols while automatically ensuring a standard level of quality to minimize adverse effects on the resulting extracellular matrix. Here we follow this idea by introducing two novel components into the current practice. First, a non-invasive method for online monitoring of resulting fluid dynamical characteristics of the coronary system is demonstrated for application during the perfusion decellularization of whole hearts. Second, the observation of the underlying rheological characteristics of the perfusates is employed to detect ongoing progress and maturation of the decellularization process. Measured data were contrasted to the respective release of specific cellular components. We demonstrate rheological measurements to be capable of detecting cellular debris along with a discriminative capture of DNA and protein ratios. We demonstrate that this perfusate biomass is well correlated to the biomass loss in the ...Continue Reading

References

Oct 27, 2010·Tissue Engineering. Part C, Methods·Allison R GilliesShyni Varghese
Mar 23, 2011·Annual Review of Biomedical Engineering·Stephen F BadylakKorkut Uygun
Apr 26, 2011·Trends in Molecular Medicine·Jeremy J Song, Harald C Ott
May 21, 2011·Tissue Engineering. Part C, Methods·Yulia ShamisEduardo Mitrani
Jan 3, 2012·Journal of Biomechanics·Colleen WitzenburgVictor H Barocas
Oct 10, 2012·Molecular and Cellular Biology·Eun Jung JangEun Sook Hwang
Apr 17, 2013·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Jörn HülsmannPayam Akhyari

❮ Previous
Next ❯

Citations

May 14, 2016·Tissue Engineering. Part C, Methods·Sharon GeertsBasak E Uygun
Feb 4, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Emma C MoranPedro M Baptista
May 9, 2012·Transplantation Proceedings·K M Park, H M Woo
May 9, 2012·Transplantation Proceedings·K M Park, H M Woo
Feb 22, 2018·Biomedical Materials·Jörn HülsmannPayam Akhyari
Aug 23, 2019·Scientific Reports·Rayssa Helena Arruda PereiraBreno Valentim Nogueira

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Yu ZhaoShuling Bai
Journal of Cellular Biochemistry
Thomas W Gilbert
Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
Hana HrebikovaJaroslav Mokry
Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova
Iu L ProtsenkoV A Abazov
© 2021 Meta ULC. All rights reserved