Rhinoviral stimuli, epithelial factors and ATP signalling contribute to bronchial smooth muscle production of IL-33

Journal of Translational Medicine
Jenny CalvénLena Uller

Abstract

Bronchial smooth muscle cells (BSMCs) from severe asthmatics have been shown to overexpress the Th2-driving and asthma-associated cytokine IL-33. However, little is known regarding factors involved in BSMC production of IL-33. Rhinovirus (RV) infections cause asthma exacerbations, which exhibit features of Th2-type inflammation. Here, we investigated the effects of epithelial-derived media and viral stimuli on IL-33 expression in human BSMCs. Primary human BSMCs from healthy (n = 3) and asthmatic (n = 3) subjects were stimulated with conditioned media from primary human bronchial epithelial cells (BECs), double-stranded (ds)RNA, dsRNA/LyoVec, or infected with RV. BSMCs were also pretreated with the purinergic receptor antagonist suramin. IL-33 expression was analysed by RT-qPCR and western blot and ATP levels were determined in cell supernatants. RV infection and activation of TLR3 by dsRNA increased IL-33 mRNA and protein in healthy and asthmatic BSMCs. These effects were inhibited by dexamethasone. BSMC expression of IL-33 was also increased by stimulation of RIG-I-like receptors using dsRNA/LyoVec. Conditioned media from BECs induced BSMC expression of IL-33, which was further enhanced by dsRNA. BEC-derived medium and viral-...Continue Reading

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Citations

Mar 10, 2016·The Journal of Allergy and Clinical Immunology·Dorothy M RyanRobert M Niven
Mar 29, 2018·Respiratory Research·Hiroyuki AizawaMasakazu Ichinose
Apr 16, 2018·The FEBS Journal·Natalia Muñoz-Wolf, Ed C Lavelle
Oct 11, 2017·Frontiers in Pharmacology·Geoffrey Burnstock
Sep 10, 2020·Frontiers in Immunology·Li Y Drake, Y S Prakash
Jun 20, 2018·Frontiers in Immunology·Katherine H RestoriElizabeth D Fixman
Feb 12, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Xiuju DaiKoji Sayama
May 1, 2021·Viruses·Shannic-Le KerrReena Ghildyal
Jul 25, 2017·The Journal of Allergy and Clinical Immunology·Philip E SilkoffSebastian L Johnston

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Methods Mentioned

BETA
transfection
protein assay
ELISA
biopsy
nuclear translocation

Software Mentioned

GraphPad Prism
Image studio Vr
Primerdesign

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