Rhipicephalus bursa Sialotranscriptomic Response to Blood Feeding and Babesia ovis Infection: Identification of Candidate Protective Antigens

Frontiers in Cellular and Infection Microbiology
Sandra AntunesAna Domingos

Abstract

Ticks are among the most prevalent blood-feeding arthropods, and they act as vectors and reservoirs for numerous pathogens. Sialotranscriptomic characterizations of tick responses to blood feeding and pathogen infections can offer new insights into the molecular interplay occurring at the tick-host-pathogen interface. In the present study, we aimed to identify and characterize Rhipicephalus bursa salivary gland (SG) genes that were differentially expressed in response to blood feeding and Babesia ovis infection. Our experimental approach consisted of RNA sequencing of SG from three different tick samples, fed-infected, fed-uninfected, and unfed-uninfected, for characterization and inter-comparison. Overall, 7,272 expressed sequence tags (ESTs) were constructed from unfed-uninfected, 13,819 ESTs from fed-uninfected, and 15,292 ESTs from fed-infected ticks. Two catalogs of transcripts that were differentially expressed in response to blood feeding and B. ovis infection were produced. Four genes coding for a putative vitellogenin-3, lachesin, a glycine rich protein, and a secreted cement protein were selected for RNA interference functional studies. A reduction of 92, 65, and 51% was observed in vitellogenin-3, secreted cement, an...Continue Reading

References

May 19, 2000·Journal of Insect Physiology·J R SauerA S Bowman
Nov 9, 2001·Annual Review of Genetics·U TepassR Fehon
Sep 26, 2002·Vaccine·Adama R TrimnellPatricia A Nuttall
Nov 14, 2002·Insect Biochemistry and Molecular Biology·C LogulloP L Oliveira
May 18, 2004·Veterinary Microbiology·José De La FuenteChristian Gortazar
Oct 12, 2004·Insect Biochemistry and Molecular Biology·Vishvanath NeneRichard Bishop
May 26, 2005·Vaccine·Adama R TrimnellPatricia A Nuttall
Jun 9, 2005·Parasitology·F Jongejan, G Uilenberg
Jul 29, 2005·Nature·Nandhini RamamoorthiErol Fikrig
Apr 11, 2006·PLoS Pathogens·Milan LabudaPatricia A Nuttall
Jun 15, 2006·Journal of Dairy Science·C BelloliG Crescenzo
Jan 9, 2007·BMC Bioinformatics·Irini A Doytchinova, Darren R Flower
Sep 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rhoel R DinglasanMarcelo Jacobs-Lorena
Dec 12, 2007·Insect Biochemistry and Molecular Biology·Ivo M B FrancischettiJosé M C Ribeiro
Apr 29, 2008·International Journal for Parasitology·K-P HunfeldJ S Gray
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Aug 30, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Damdinsuren BoldbaatarKozo Fujisaki
Feb 3, 2009·Journal of Insect Physiology·Kevin V DonohueR Michael Roe
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Ivo M B FrancischettiJose M C Ribeiro
Jun 26, 2010·Journal of Insect Physiology·Damdinsuren BoldbaatarKozo Fujisaki
Jul 20, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S HarrusG Baneth
Jul 24, 2010·BMC Genomics·Elen AnatrielloBeatriz R Ferreira
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Feb 9, 2011·Experimental & Applied Acarology·Sandra Eloisi DenardiMaria Izabel Camargo-Mathias
Apr 26, 2011·International Journal for Parasitology·Ricardo F Mercado-CurielKelly A Brayton
Mar 1, 2010·Ticks and Tick-borne Diseases·W Reuben Kaufman
Aug 20, 2011·Journal of Proteomics·Ivo M B FrancischettiJosé M C Ribeiro
Oct 7, 2011·Parasitology Research·Shahrokh Ranjbar-BahadoriParviz Shayan
Nov 2, 2011·The Journal of Cell Biology·Matteo Bonazzi, Pascale Cossart

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Citations

Apr 9, 2020·Biomolecules·Margarita VillarJosé de la Fuente
Feb 11, 2020·Frontiers in Cellular and Infection Microbiology·Poliana Fernanda GiachettoRenato Andreotti
Nov 3, 2020·Parasite Immunology·Larissa Almeida MartinsMichail Kotsyfakis
Dec 29, 2020·Parasite Immunology·Charles Edward Hart, Saravanan Thangamani
Feb 6, 2021·PLoS Neglected Tropical Diseases·Ana OleagaRicardo Pérez-Sánchez
Apr 20, 2021·Heliyon·Iván PachecoJosé de la Fuente
Dec 10, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Larissa Almeida MartinsMichail Kotsyfakis

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Datasets Mentioned

BETA
SRR4428987
PRJNA348674
GFZK00000000

Methods Mentioned

BETA
PCR
RNA-Seq
cDNA library construction
gene knockdown

Software Mentioned

Primer3
Excel
Blast2GO
StabVida
VaxiJen Server
geNorm
[UNK]
UniProt
Oases
BLAST

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