Rhizobacter gummiphilus NS21 has two rubber oxygenases (RoxA and RoxB) acting synergistically in rubber utilisation.

Applied Microbiology and Biotechnology
Jakob BirkeDieter Jendrossek

Abstract

Biodegradation of poly(cis-1,4-isoprene) (rubber) by Gram-negative bacteria has been investigated on the enzymatic level only in Steroidobacter cummioxidans 35Y (previously Xanthomonas sp. 35Y). This species produces two kinds of rubber oxygenases, RoxA35Y and RoxB35Y, one of which (RoxB35Y) cleaves polyisoprene to a mixture of C20- and higher oligoisoprenoids while the other (RoxA35Y) cleaves polyisoprene and RoxB35Y-derived oligoisoprenoids to the C15-oligoisoprenoid 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (ODTD). ODTD can be taken up by S. cummioxidans and used as a carbon source. Gram-positive rubber-degrading bacteria employ another type of rubber oxygenase, latex clearing protein (Lcp), for the initial oxidative attack of the polyisoprene molecule. In this contribution, we examined which type of rubber oxygenase is present in the only other well-documented Gram-negative rubber-degrading species, Rhizobacter gummiphilus NS21. No homologue for an Lcp protein but homologues for a putative RoxA and a RoxB protein (the latter identical to a previously postulated LatA-denominated rubber cleaving enzyme) were identified in the genome of strain NS21. The roxANS21 and roxBNS21 genes were separately expressed in a ∆roxA35Y/∆roxB3...Continue Reading

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Citations

Nov 1, 2018·Applied Microbiology and Biotechnology·Dieter Jendrossek, Jakob Birke
Aug 24, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Stefania Pegorin BragaRenata Castiglioni Pascon
Sep 6, 2019·Applied Microbiology and Biotechnology·Jakob Birke, Dieter Jendrossek
Mar 3, 2020·Bioscience, Biotechnology, and Biochemistry·Daisuke Kasai
Jul 19, 2020·Applied Microbiology and Biotechnology·Namiko GibuDaisuke Kasai
Jul 3, 2021·Polymers·Ann Anni BasikKumar Sudesh

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Methods Mentioned

BETA
PCR
ion exchange chromatography
gel filtration
electrophoresis

Software Mentioned

ExPASy pI / Mw

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