Rhizobial NodL O-acetyl transferase and NodS N-methyl transferase functionally interfere in production of modified Nod factors

Journal of Bacteriology
I M López-LaraJ E Thomas-Oates

Abstract

The products of the rhizobial nodulation genes are involved in the biosynthesis of lipochitin oligosaccharides (LCOs), which are host-specific signal molecules required for nodule formation. The presence of an O-acetyl group on C-6 of the nonreducing N-acetylglucosamine residue of LCOs is due to the enzymatic activity of NodL. Here we show that transfer of the nodL gene into four rhizobial species that all normally produce LCOs that are not modified on C-6 of the nonreducing terminal residue results in production of LCOs, the majority of which have an acetyl residue substituted on C-6. Surprisingly, in transconjugant strains of Mesorhizobium loti, Rhizobium etli, and Rhizobium tropici carrying nodL, such acetylation of LCOs prevents the endogenous nodS-dependent transfer of the N-methyl group that is found as a substituent of the acylated nitrogen atom. To study this interference between nodL and nodS, we have cloned the nodS gene of M. loti and used its product in in vitro experiments in combination with purified NodL protein. It has previously been shown that a chitooligosaccharide N deacetylated on the nonreducing terminus (the so-called NodBC metabolite) is the preferred substrate for NodS as well as for NodL. Here we show ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Nov 25, 1990·Nucleic Acids Research·J M Collins-EmersonD B Scott
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Apr 1, 1984·Journal of Bacteriology·K D NoelM A Cevallos
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Sep 29, 1995·The Journal of Biological Chemistry·S JabbouriW J Broughton
Apr 1, 1996·Molecular Plant-microbe Interactions : MPMI·J L Folch-MallolM Megías
Nov 19, 1997·Molecular Microbiology·P MergaertM Holsters
Mar 21, 1998·The Journal of Biological Chemistry·P J SutherlandN P Price
May 22, 1998·Sub-cellular Biochemistry·E KamstD Kafetzopoulos
Mar 6, 1999·Current Opinion in Plant Biology·M R Bladergroen, H P Spaink
Jan 1, 1989·Plant Molecular Biology·H P SpainkB E Lugtenberg

❮ Previous
Next ❯

Citations

Jun 7, 2012·Molecular Plant-microbe Interactions : MPMI·Rik H M Op den CampRené Geurts
Jan 28, 2012·Acta Crystallographica. Section D, Biological Crystallography·Krzysztof BrzezinskiMariusz Jaskolski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Chemical Communications : Chem Comm
Karen T HøjholtPablo Beato
Chemical Communications : Chem Comm
Dan LiHuanting Wang
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Ozgur CakiciMariusz Jaskolski
PM & R : the Journal of Injury, Function, and Rehabilitation
Chad CookJames A Nunley
© 2021 Meta ULC. All rights reserved