PMID: 2115865Aug 1, 1990Paper

Rhizobium meliloti Fix L is an oxygen sensor and regulates R. meliloti nifA and fixK genes differently in Escherichia coli

Journal of Bacteriology
P de PhilipP Boistard

Abstract

In Rhizobium meliloti, nif and fix genes, involved in nitrogen fixation during symbiosis with alfalfa, are under the control of two transcriptional regulators encoded by nifA and fixK. Expression of nifA and fixK is under the control of FixL/J, a two-component regulatory system. We showed, using Escherichia coli as a heterologous host, that FixL/J controls nifA and fixK expression in response to microaerobiosis. Furthermore, expression of the sensor gene fixL and of the activator gene fixJ under the control of two different promoters allowed us to show that FixL mediates microaerobic induction of nifA when the level of FixJ is low and aerobic repression of nifA when the level of FixJ is high. Similarly, activation of fixK occurred in microaerobiosis when the FixJ level was low in the presence of FixL. In contrast to nifA, fixK expression was not affected by FixL in aerated cultures when the level of FixJ was high. We conclude that R. meliloti FixL senses oxygen in the heterologous host E. coli consistent with the microaerobic induction of nifA and fixK in R. meliloti and that nifA and fixK promoters are differentially activated by FixJ in response to the oxygen signal.

References

Jul 1, 1987·Journal of Bacteriology·G DittaC H Kim
Dec 1, 1989·Microbiological Reviews·J B StockA M Stock
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K A BorkovichM I Simon
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S ForstM Inouye
Jan 1, 1989·Annual Review of Genetics·L M AlbrightF M Ausubel
Jan 1, 1988·Journal of Bacteriology·J M SlauchT J Silhavy
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E L VirtsG S Ditta
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A J Ninfa, B Magasanik
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S Iuchi, E C Lin
Dec 9, 1985·Nucleic Acids Research·M M WayeG Winter
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S C WinansE W Nester
Aug 1, 1988·Journal of Bacteriology·L A Weston, R J Kadner
Apr 8, 1988·Cell·J S Parkinson
Aug 1, 1987·Journal of Bacteriology·M J Friedrich, R J Kadner
Jan 1, 1986·Annual Review of Genetics·G N GussinF M Ausubel
Jan 1, 1982·Annual Review of Genetics·B Magasanik
Apr 1, 1984·Cell·W W SzetoF M Ausubel

❮ Previous
Next ❯

Citations

Nov 1, 1995·Molecular & General Genetics : MGG·I D'hoogheJ Vanderleyden
May 5, 2000·Molecular Plant-microbe Interactions : MPMI·D CabanesJ Batut
Jun 15, 2011·Annual Review of Microbiology·Jonathan T Henry, Sean Crosson
Jan 31, 2018·Nature Reviews. Microbiology·Philip PooleJason Terpolilli
Mar 1, 1991·Molecular Microbiology·P A Kaminski, C Elmerich
Oct 1, 1992·Molecular & General Genetics : MGG·P de PhilipP Boistard
Nov 5, 1999·The Journal of Biological Chemistry·A M GarneroneJ Batut
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chang Fu TianJacques Batut
Jul 1, 1993·World Journal of Microbiology & Biotechnology·M MegíasC Sousa
Jan 1, 1994·Antonie van Leeuwenhoek·J Batut, P Boistard
Nov 17, 1999·Molecular Microbiology·S Da ReD Kahn
Jun 4, 2019·Frontiers in Plant Science·Chiara PucciarielloPierdomenico Perata
Nov 5, 1999·Annual Review of Microbiology·C E BauerT H Bird
Nov 22, 2008·Antioxidants & Redox Signaling·F Wayne Outten, Elizabeth C Theil
Jun 13, 2000·Journal of Bacteriology·D CabanesJ Batut
Feb 27, 2021·Frontiers in Microbiology·Ilenne Del ValleLauren B Stadler
Jan 19, 2008·Biochemistry·James D SatterleeRalph Jimenez
Jul 1, 1995·Respiration Physiology·J Fandrey
Jun 1, 1992·Journal of Bacteriology·P G AgronD R Helinski
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·B L Taylor, I B Zhulin
Jun 1, 1994·Research in Microbiology·P G AgronD R Helinski
Nov 1, 1993·Journal of Bacteriology·J M ReyratJ Batut
Dec 1, 1995·Journal of Bacteriology·A I LorochR A Ludwig
Mar 10, 2004·Applied and Environmental Microbiology·Martine MorisJan Michiels
Sep 1, 1994·Microbiological Reviews·H M Fischer
Jun 9, 2005·Protein Expression and Purification·Christine SuquetJames D Satterlee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.