PMID: 1738Jul 1, 1975

Rhizopus microsporus strain UzLT-I--a thermotolerant producer of lipase

Prikladnaia biokhimiia i mikrobiologiia
M Z ZakirovK Davranov

Abstract

The properties of the thermotolerant fungus Rhizopus microsporus strain UzLT-1--producer of lipolyptic enzymes are described. Optimal cultivation conditions--40 degrees, C, pH 4.5--Are determined. The lipolytic activity of the culture on the medium consisting of corn extract (2%), cotton-seed oil (1%) and water is 850 ml 0.1 n KOH per 100 ml culture liquid. The enzymic preparations of lipase have been precipitated by isopropanol and ammonium sulphate. The preparation precipitated by isopropanol shows its macimum activity at pH 4.2 and 7.8 and a temperature of 40--50 degrees C.

Related Concepts

Hydrogen-Ion Concentration
Exolipase
Rhizopus

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