PMID: 9435067Jan 22, 1998Paper

Rhizoremediation of trichloroethylene by a recombinant, root-colonizing Pseudomonas fluorescens strain expressing toluene ortho-monooxygenase constitutively

Applied and Environmental Microbiology
D C YeeThomas K Wood

Abstract

Trichloroethylene (TCE) was removed from soils by using a wheat rhizosphere established by coating seeds with a recombinant, TCE-degrading Pseudomonas fluorescens strain that expresses the tomA+ (toluene o-monooxygenase) genes from Burkholderia cepacia PR1(23)(TOM23C). A transposon integration vector was used to insert tomA+ into the chromosome of P. fluorescens 2-79, producing a stable strain that expressed constitutively the monooxygenase at a level of 1.1 nmol/min.mg of protein (initial TCE concentration, 10 microM, assuming that all of the TCE was in the liquid) for more than 280 cell generations (36 days). We also constructed a salicylate-inducible P. fluorescens strain that degraded TCE at an initial rate of 2.6 nmol/min.mg of protein in the presence of 10 microM TCE [cf. B. cepacia G4 PR1(23) (TOM23C), which degraded TCE at an initial rate of 2.5 nmol/min.mg of protein]. A constitutive strain, P. fluorescens 2-79TOM, grew (maximum specific growth rate, 0.78 h-1) and colonized wheat (3 x 10(6) CFU/cm of root) as well as wild-type P. fluorescens 2-79 (maximum specific growth rate, 0.77 h-1; level of colonization, 4 x 10(6) CFU/cm of root). Rhizoremediation of TCE was demonstrated by using microcosms containing the constitu...Continue Reading

References

Dec 1, 1992·Applied and Environmental Microbiology·M S Shields, M J Reagin
Jul 1, 1992·Applied and Environmental Microbiology·D W GrahamR G Arnold
May 1, 1990·Applied and Environmental Microbiology·B R FolsomP H Pritchard
Dec 25, 1989·Nucleic Acids Research·A W Smith, B H Iglewski
Jan 1, 1989·Critical Reviews in Toxicology·J V BrucknerJ N Blancato
May 1, 1987·Applied and Environmental Microbiology·M J NelsonP H Pritchard
Apr 1, 1995·Applied and Environmental Microbiology·M S ShieldsC Somerville
Aug 1, 1996·Applied and Environmental Microbiology·K McClayR J Steffan
Mar 1, 1983·Applied and Environmental Microbiology·L P Wackett, D T Gibson
Mar 1, 1983·Applied and Environmental Microbiology·B R Reddy, N Sethunathan
Aug 1, 1986·Applied and Environmental Microbiology·M J NelsonP H Pritchard
Jun 1, 1908·The Journal of Hygiene·A T Macconkey
Feb 1, 1995·Environmental Science & Technology·L P Wackett, D L Allan

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Citations

Feb 8, 2006·Applied and Environmental Microbiology·Cindy H WuWilfred Chen
Dec 8, 2005·Applied and Environmental Microbiology·Mariela R MontiCarlos E Argaraña
Apr 30, 2009·Environmental Science and Pollution Research International·Nele WeyensJaco Vangronsveld
Jun 21, 2011·Applied and Environmental Microbiology·Xiaoxue Wang, Thomas K Wood
Oct 13, 2012·Critical Reviews in Biotechnology·Awadhesh Kumar ShuklaSuresh Kumar Dubey
Feb 12, 2015·Lab on a Chip·David SanahujaXavier Muñoz-Berbel
Apr 5, 2005·Applied Microbiology and Biotechnology·JiHyeon Song, Kerry A Kinney
Jan 13, 2004·Molecular Plant-microbe Interactions : MPMI·Irene KuiperBen J J Lugtenberg
Sep 11, 2009·International Journal of Environmental Research and Public Health·Martina McGuinness, David Dowling
Jul 25, 2006·Biotechnology and Bioengineering·Wonkyu LeeWilfred Chen
Oct 16, 2019·Cellular and Molecular Life Sciences : CMLS·Shengbo WuJianjun Qiao
Sep 22, 2007·Applied Microbiology and Biotechnology·Arno ReinUlrich Gosewinkel Karlson
Aug 23, 2006·Critical Reviews in Biotechnology·Meltem Urgun-DemirtasKrishna Pagilla
Jun 18, 2005·Letters in Applied Microbiology·L A O'Sullivan, E Mahenthiralingam
Dec 20, 2015·International Journal of Phytoremediation·Philipp SchöftnerThomas G Reichenauer
Jul 20, 2005·Biotechnology and Bioengineering·Thammajun LeungsakulThomas K Wood
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Oct 31, 2000·Applied and Environmental Microbiology·H ShimT K Wood
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Aug 11, 2021·Viruses·Philip Lauman, Jonathan J Dennis
Jul 25, 2017·Environmental Science & Technology·Sharon L DotyMichael J Blaylock
May 22, 2007·BMC Microbiology·Jintae LeeThomas K Wood
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Jul 19, 2011·Trends in Biotechnology·Juan-Luis RamosRegina-Michaela Wittich

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